Indomethacin disrupts the protective effect of phosphatidylcholine against bile salt-induced ileal mucosa injury
| Autor: | N. G. Venneman, Lma Akkermans, Alfons B.A. Kroese, J. E. Van Dijk, M. Petruzzelli, A. Verheem, K. J. van Erpecum |
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| Rok vydání: | 2006 |
| Předmět: |
Cholagogues and Choleretics
medicine.medical_specialty Indomethacin Clinical Biochemistry Ileum Biochemistry Permeability Lesion chemistry.chemical_compound Indometacin Phosphatidylcholine Internal medicine medicine Animals Humans Intestinal Mucosa Rats Wistar Cytotoxicity Incubation Taurodeoxycholic Acid L-Lactate Dehydrogenase biology Anti-Inflammatory Agents Non-Steroidal digestive oral and skin physiology General Medicine Rats medicine.anatomical_structure Endocrinology chemistry Enzyme inhibitor Tocolytic Phosphatidylcholines biology.protein Female Caco-2 Cells medicine.symptom medicine.drug |
| Zdroj: | European Journal of Clinical Investigation. 36:105-112 |
| ISSN: | 1365-2362 0014-2972 |
| DOI: | 10.1111/j.1365-2362.2006.01595.x |
| Popis: | Background Indomethacin (Indo) exerts local toxic effects on small intestinal mucosa, possibly in association with hydrophobic bile salts. We investigated the potential toxic effects of Indo on ileal mucosa and the role of phosphatidylcholine (PC). Materials and methods Transmucosal resistance and Na-fluorescein permeability of ileal mucosa segments from female Wistar rats were determined in Ussing chambers during a 30-min incubation with model systems containing: control-buffer, taurodeoxycholate (TDC), Indo, TDC-Indo, TDC-PC, or TDC-PC-Indo. Decrease of resistance and increase of permeability were considered as parameters for mucosal injury. After incubation in Ussing chambers, the histopathology was examined to quantify the extent of mucosal injury. Also, in CaCo-2 cells, LDH-release was determined as a measure of cytotoxicity, after incubation with various model systems. Results Decrease of resistance and increase of permeability were highest in systems containing TDC-Indo (P |
| Databáze: | OpenAIRE |
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