A double base change in alternate base pairs induced by ultraviolet irradiation in a glycine transfer RNA gene
| Autor: | Charles W. Hill, Robert W. Dunst, Robert D. Coleman |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
DNA
Bacterial Base Composition Base Sequence Base pair Ultraviolet Rays Glycine Dose-Response Relationship Radiation Biology medicine.disease_cause Molecular biology Base change Genes RNA Transfer Transfer RNA Mutation (genetic algorithm) Genetics medicine Escherichia coli Missense mutation Threonine Molecular Biology Gene |
| Zdroj: | Moleculargeneral genetics : MGG. 177(2) |
| ISSN: | 0026-8925 |
| Popis: | The glyUsu AGA mutation affects Escherichia coli tRNA GGG G1 y , changing it to an AGA missense suppressor tRNA. Sequence studies have shown that the mutation involves a double base substitution at the first and third positions of the tRNA anticodon, the result being a change in the anticodon from CCC to UCU. A system has been developed to facilitate the detection of this novel mutation, and we have shown that ultraviolet irradiation and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) are effective in causing the double base change. A single observation of the mutation occurring spontaneously has been made also. The frequency of MNNG-induced glyUsu AGA mutations is compatible with their being caused by two separate mutagenic events. The frequency of UV-induced glyUsu AGA mutations, however, strongly suggests that the occurrence of one base substitution strongly enhances the chance of finding the second substitution at the alternate position. In addition to the double change in the anticodon, the glyUsu AGA tRNA differs from tRNA GGG G1 y in that it bears a modification of the A adjacent to the 3′ position of the anticodon. Most likely, this modified base is N-[9-(β-D-ribofuranosyl)-purin-6-ylcarbamoyl] threonine. |
| Databáze: | OpenAIRE |
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