Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva
| Autor: | Masahiro Muraguchi, Gota Cho, Fusako Iwata, Jinsen Kang, Xuejun Li, Ko Okamura, Yasuko Ishikawa, Keiko Ooga, Yasukazu Ohmoto, Di Wang, Masaaki Takai, Yan Pan, Masayuki Shono, Toyoki Mori |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Agonist Quinuclidines medicine.medical_specialty Saliva medicine.drug_class Biophysics Fluorescent Antibody Technique Thiophenes Biochemistry Salivary Glands Young Adult chemistry.chemical_compound Cevimeline Membrane Microdomains stomatognathic system Internal medicine Muscarinic acetylcholine receptor medicine Animals Humans Parotid Gland Amylase Rats Wistar Wakefulness Molecular Biology Lipid raft Aged Aged 80 and over Receptor Muscarinic M3 Microscopy Confocal biology Mucin Age Factors Middle Aged Aquaporin 5 Circadian Rhythm Rats Endocrinology chemistry Amylases biology.protein Female Lysozyme Sleep medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1790:49-56 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2008.08.009 |
| Popis: | Background It is unknown whether AQP5 and lipid rafts are released into human unstimulated (resting) saliva and saliva in response to secretagogues. Methods In order to quantitate the salivary concentration of AQP5, we produced a polyclonal antibody for human AQP5 and developed an enzyme-like immunosorbent assay (ELISA). Results AQP5 and lipid rafts were identified in human resting saliva. The amount of AQP5 in resting saliva showed a diurnal variation with high levels during waking hours, and an age-related decrease in AQP5 was coincident with the volume of resting saliva. Cevimeline, a muscarinic acetylcholine receptor (mAChR) agonist, induced the release of AQP5 with lipid rafts, amylase, mucin, and lysozyme. Changes in saliva AQP5 levels after cevimeline administration occurred simultaneously with changes in saliva flow rates. Confocal microscopy revealed that AQP5 was located in the apical plasma membrane and showed a diffuse pattern in parotid glands under resting conditions. Following cevimeline administration, AQP5 was predominantly associated with the APM and was localized in the lumen. General significance AQP5 and lipid rafts were released with salivary proteins from human salivary glands by the stimulation of M 3 mAChRs, and that changes in saliva AQP5 levels can be used as an indicator of salivary flow rate and also as a useful index of M 3 mAChR agonist's action on human salivary glands. |
| Databáze: | OpenAIRE |
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