Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds
| Autor: | Pooria Sharif-Kashani, Suxian Huang, Tatiana Segura, Pirouz Kavehpour, Yuguo Lei, Yong Chen |
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| Rok vydání: | 2010 |
| Předmět: |
Scaffold
Materials science Genetic enhancement Biophysics Neovascularization Physiologic Nanoparticle Bioengineering macromolecular substances Polyethylene glycol Gene delivery Hydrogel Polyethylene Glycol Dimethacrylate Article Polyethylene Glycols Biomaterials chemistry.chemical_compound Cations Hyaluronic acid PEG ratio Animals Humans Polyethyleneimine Microscopy Confocal Tissue Scaffolds Gene Transfer Techniques technology industry and agriculture DNA Freeze Drying chemistry Biochemistry Mechanics of Materials Self-healing hydrogels Ceramics and Composites Nanoparticles Cattle Chickens |
| Zdroj: | Biomaterials. 31:9106-9116 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2010.08.016 |
| Popis: | The effective and sustained delivery of DNA and siRNAs locally would increase the applicability of gene therapy in tissue regeneration and cancer therapy. One promising approach is to use hydrogel scaffolds to encapsulate and deliver nucleotides in the form of nanoparticles to the disease sites. However, this approach is currently limited by the inability to load concentrated and active gene delivery nanoparticles into the hydrogels due to the severe nanoparticle aggregation during the loading process. Here, we present a process to load concentrated and un-aggregated non-viral gene delivery nanoparticles, using DNA/polyethylene imine (PEI) polyplexes as an example, into neutral polyethylene glycol (PEG), negatively charged hyaluronic acid (HA) and protein fibrin hydrogels crosslinked through various chemistries. The encapsulated polyplexes are highly active both in vitro and in vivo. We believe this process will significantly advance the applications of hydrogel scaffold mediated non-viral gene delivery in tissue regeneration and cancer therapy. |
| Databáze: | OpenAIRE |
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