The UCMSC-bFGF/Scaffold System Accelerates the Healing of the Uterine Full-Thickness Injury

Autor: Limin, Wang, Simin, Yao, Feifei, Huang, Haining, Lv, Dan, Liu, Tianyun, Gao, Bin, Wang, Zhenhua, Zhou, Chenrui, Cao, Qi, Zhu, Qiao, Weng, Guangfeng, Zhao, Yali, Hu
Rok vydání: 2023
Předmět:
Zdroj: Tissue Engineering Part A. 29:112-125
ISSN: 1937-335X
1937-3341
Popis: Severe uterine injury is a major cause of endometrial scar formation and female infertility. Currently, the methods for accelerating injured uterine healing are still lacking. Genetic engineering modification of mesenchymal stem cells (MSCs) has been shown great promise in preclinical studies on regeneration. Here, we constructed a type of UC-MSCs with overexpressed bFGF (UC-MSCbFGF) and investigated the effects of the UC-MSCbFGF/scaffold on functional regeneration of the full-thickness defect uterus of the rat model. At day 7, 14, 30 after treatments, the rats were euthanized and the injured uterus were observed. The structural and functional change of uterine were assessed by hematoxylin and eosin (HE) staining, immunohistochemical staining and fertility experiment. The UC-MSCbFGF/scaffold group exhibited anti-inflammatory effect, and the number of CD45+ cell in the UC-MSCbFGF/scaffold group was significantly less than that in UC-MSCs/scaffold group and scaffold group, but higher than sham-operated group at day 7 post-mending. At day 14, the UC-MSCbFGF/scaffold group exhibited dramatically pro-angiogenesis efficacy compared with UC-MSCs/scaffold group and scaffold group. At day 30, the endometrial thickness, structure of myometrium and blood vessels in the UC-MSCbFGF/scaffold were better than those of the UC-MSCs/scaffold group and scaffold group, even close to sham-operated group. Implantation rate at injury region post-operation 30 days in the UC-MSCbFGF/scaffold group (8/16) was significantly higher than that in UC-MSCs/scaffold group (1/16) and scaffold group (0/16). Taken together, the UC-MSCbFGF/scaffold system suppressed local inflammation, promoted angiogenesis and accelerated regeneration of the defected uterine wall, and thereby greatly shortened the healing time of the injured uterus.
Databáze: OpenAIRE
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