T-brainhomologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo
| Autor: | Daisuke Kurokawa, Koji Akasaka, Takuya Fuchikami, Nori Satoh, Miho Kataoka, Keiko Mitsunaga-Nakatsubo, Toshiya Hosomi, Hiraku Shimada, Naoaki Sakamoto, Kazuko Takata, Taishin Shimotori, Takashi Watanabe, Takashi Yamamoto, Shinichiro Kusunoki, Yoshito Harada, Shonan Amemiya |
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| Rok vydání: | 2002 |
| Předmět: |
Embryo
Nonmammalian animal structures Morpholino Mesenchyme Molecular Sequence Data Ectoderm Ion Pumps Mice Hemicentrotus Multienzyme Complexes medicine Animals Amino Acid Sequence Molecular Biology beta Catenin Cell Nucleus Embryonic Induction Base Sequence Sequence Homology Amino Acid biology Arsenite Transporting ATPases Gene Expression Regulation Developmental Gastrula Anatomy Blastula biology.organism_classification Cell biology Gastrulation Cytoskeletal Proteins medicine.anatomical_structure Protein Biosynthesis Sea Urchins embryonic structures Trans-Activators Endoderm T-Box Domain Proteins Archenteron Signal Transduction Transcription Factors Developmental Biology |
| Zdroj: | Development. 129:5205-5216 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Signals from micromere descendants play a crucial role in sea urchin development. In this study, we demonstrate that these micromere descendants express HpTb , a T-brain homolog of Hemicentrotus pulcherrimus. HpTb is expressed transiently from the hatched blastula stage through the mesenchyme blastula stage to the gastrula stage. By a combination of embryo microsurgery and antisense morpholino experiments, we show that HpTb is involved in the production of archenteron induction signals. However, HpTb is not involved in the production of signals responsible for the specification of secondary mesenchyme cells, the initial specification of primary mesenchyme cells, or the specification of endoderm. HpTb expression is controlled by nuclear localization of β -catenin, suggesting that HpTb is in a downstream component of the Wnt signaling cascade. We also propose the possibility that HpTb is involved in the cascade responsible for the production of signals required for the spicule formation as well as signals from the vegetal hemisphere required for the differentiation of aboral ectoderm. |
| Databáze: | OpenAIRE |
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