A mitochondria targeting Mn nanoassembly of BODIPY for LDH-A, mitochondria modulated therapy and bimodal imaging of cancer
| Autor: | Qiu-Yun Chen, Jing Gao, Daniel Boison, Yao Zhao, Qin-Mei Xu, Tao Huang, Huang Yang, Wen-Long Lu |
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| Rok vydání: | 2016 |
| Předmět: |
Boron Compounds
Diagnostic Imaging 0301 basic medicine Fluorescence-lifetime imaging microscopy Apoptosis Caspase 3 Biology Mitochondrion 010402 general chemistry 01 natural sciences Mice 03 medical and health sciences Colloid and Surface Chemistry In vivo Neoplasms Tumor Cells Cultured medicine Animals Humans Physical and Theoretical Chemistry Cell Proliferation Fluorescent Dyes Membrane Potential Mitochondrial Mice Inbred ICR L-Lactate Dehydrogenase Cell growth Cancer Surfaces and Interfaces General Medicine medicine.disease Xenograft Model Antitumor Assays Mitochondria 0104 chemical sciences Cell biology Isoenzymes 030104 developmental biology Cancer cell Female Lactate Dehydrogenase 5 Signal Transduction Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 147:387-396 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2016.08.024 |
| Popis: | HIF-1α and LDH-A are important targets for hypoxia-driven drug resistance. Mitochondria targeted fluorescent manganese(II)-complexes can be used as potential fluorescence imaging agents, MRI contrast agents and HIF-1α and LDH-A involved anticancer complexes. In this study, a fluorescent manganese(II) nanoparticle, labeled as (PEG-Mn-BDA), was synthesized and used as both fluorescent and MRI imaging agents in cancer cells. In vitro bioassay results indicate that PEG-Mn-BDA was able to inhibit LDH-A activity and depolarize mitochondrial membrane potential with the generation of intracellular ROS, which contributed to the induction of apoptosis. Moreover, the pro-apoptotic protein, caspase 3 was highly expressed. In vivo, PEG-Mn-BDA could also exert inhibition on a mouse hepatocellular carcinoma xenograft. These results suggest that mitochondria targeted PEG-Mn-BDA was able to simultaneously induce selective inhibition on cancer cells and a mouse carcinoma xenograft, label cancer cells with fluorescence and enhance MRI contrast. Therefore, PEG-Mn-BDA is a good candidate for cancer treatment and imaging. |
| Databáze: | OpenAIRE |
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