Ect2, an ortholog of Drosophila Pebble, regulates formation of growth cones in primary cortical neurons
| Autor: | Takahiro Tsuji, Yoshihiko Aoki, Mitsuko Dohmoto, Mohammad Saharul Islam, Chiharu Higashida, Haruhiro Higashida |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Neurite
Cellular differentiation Biology Article Cellular and Molecular Neuroscience RNA interference medicine Animals Drosophila Proteins Guanine Nucleotide Exchange Factors Progenitor cell Axon GEF Growth cone Neurons Cell Differentiation Growth cones Cell Biology Cerebral cortex Immunohistochemistry Embryonic stem cell Cell biology medicine.anatomical_structure nervous system Neuronal development Drosophila RNA Interference Neuroscience Drosophila Protein |
| Zdroj: | Neurochemistry International. 61(6):854-858 |
| ISSN: | 0197-0186 |
| Popis: | In collaboration with Marshall Nirenberg, we performed in vivo RNA interference (RNAi) genome-wide screening in Drosophila embryos. Pebble has been shown to be involved in Drosophila neuronal development. We have also reported that depletion of Ect2, a mammalian ortholog of Pebble, induces differentiation in NG108-15 neuronal cells. However, the precise role of Ect2 in neuronal development has yet to be studied. Here, we confirmed in PC12 pheochromocytoma cells that inhibition of Ect2 expression by RNAi stimulated neurite outgrowth, and in the mouse embryonic cortex that Ect2 was accumulated throughout the ventricular and subventricular zones with neuronal progenitor cells. Next, the effects of Ect2 depletion were studied in primary cultures of mouse embryonic cortical neurons: Loss of Ect2 did not affect the differentiation stages of neuritogenesis, the number of neurites, or axon length, while the numbers of growth cones and growth cone-like structures were increased. Taken together, our results suggest that Ect2 contributes to neuronal morphological differentiation through regulation of growth cone dynamics. © 2011 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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