Enhanced bioadhesivity of dopamine-functionalized polysaccharidic membranes for general surgery applications

Autor: Andrea Travan, Joanna W. A. M. Bosmans, Nicole D. Bouvy, L. Perge, Ivan Donati, Marie-Pierre Foulc, Massimiliano Borgogna, Sergio Paoletti, Eleonora Marsich, Francesca Scognamiglio
Přispěvatelé: Scognamiglio, Francesca, Travan, Andrea, Borgogna, MASSIMILIANO ANTONIO, Donati, Ivan, Marsich, Eleonora, Bosmans, J. W. A. M., Perge, L., Foulc, M. P., Bouvy, N. D., Paoletti, Sergio, Promovendi NTM, RS: NUTRIM - R1 - Metabolic Syndrome, Surgery, MUMC+: MA Heelkunde (9)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Dopamine
Sus scrofa
Biocompatible Materials
02 engineering and technology
Alginate
Bioadhesion
Functionalized polysaccharides
Membranes
01 natural sciences
Biochemistry
Mice
Glucuronic Acid
Spectroscopy
Fourier Transform Infrared
chemistry.chemical_classification
Hexuronic Acids
Biomaterial
Adhesiveness
General Medicine
Adhesion
021001 nanoscience & nanotechnology
Functionalized polysaccharide
Membrane
Surgical Procedures
Operative
0210 nano-technology
Biotechnology
Materials science
Alginates
Cell Survival
Biomedical Engineering
010402 general chemistry
Polysaccharide
Biomaterials
In vivo
Polysaccharides
Animals
Humans
Molecular Biology
Mechanical Phenomena
Wound Healing
Water
Membranes
Artificial
Fibroblasts
In vitro
0104 chemical sciences
chemistry
NIH 3T3 Cells
Adhesive
Wound healing
Biomedical engineering
Zdroj: Acta Biomaterialia, 44, 232-242. Elsevier / Bunge
ISSN: 1878-7568
1742-7061
Popis: An emerging strategy to improve adhesiveness of biomaterials in wet conditions takes inspiration from the adhesive features of marine mussel, which reside in the chemical reactivity of catechols. In this work, a catechol-bearing molecule (dopamine) was chemically grafted onto alginate to develop a polysaccharide-based membrane with improved adhesive properties. The dopamine-modified alginates were characterized by NMR, UV spectroscopy and in vitro biocompatibility. Mechanical tests and in vitro adhesion studies pointed out the effects of the grafted dopamine within the membranes. The release of HA from these resorbable membranes was shown to stimulate fibroblasts activities (in vitro). Finally, a preliminary in vivo test was performed to evaluate the adhesiveness of the membrane on porcine intestine (serosa). Overall, this functionalized membrane was shown to be biocompatible and to possess considerable adhesive properties owing to the presence of dopamine residues grafted on the alginate backbone. Statement of Significance This article describes the development of a mussels-inspired strategy for the development of an adhesive polysaccharide-based membrane for wound healing applications. Bioadhesion was achieved by grafting dopamine moieties on the structural component on the membrane (alginate): this novel biomaterial showed improved adhesiveness to the intestinal tissue, which was demonstrated by both in vitro and in vivo studies. Overall, this study points out how this nature-inspired strategy may be successfully exploited for the development of novel engineered biomaterials with enhanced bioadhesion, thus opening for novel applications in the field of general surgery.
Databáze: OpenAIRE
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