Nanometric molecular separation measurements by single molecule photobleaching
| Autor: | Daniel J. Rolfe, Marisa L. Martin-Fernandez, Laura C. Zanetti-Domingues, Michael Hirsch, Stephen E. D. Webb, Kathrin M. Scherer, Selene K. Roberts, Benjamin C. Coles, Sarah R. Needham, Christopher J. Tynan |
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| Rok vydání: | 2015 |
| Předmět: |
Photobleaching
Fluorophore Macromolecular Substances Nanotechnology Fluorescence General Biochemistry Genetics and Molecular Biology Molecular Imaging ErbB Receptors chemistry.chemical_compound Förster resonance energy transfer Microscopy Fluorescence chemistry Cricetinae Microscopy Animals Humans Molecule Female Molecular imaging Molecular Biology Fluorescent Dyes Macromolecule |
| Zdroj: | Methods. 88:76-80 |
| ISSN: | 1046-2023 |
| Popis: | Although considerable progress has been made in imaging distances in cells below the diffraction limit using FRET and super-resolution microscopy, methods for determining the separation of macromolecules in the 10-50 nm range have been elusive. We have developed fluorophore localisation imaging with photobleaching (FLImP), based on the quantised bleaching of individual protein-bound dye molecules, to quantitate the molecular separations in oligomers and nanoscale clusters. We demonstrate the benefits of using our method in studying the nanometric organisation of the epidermal growth factor receptor in cells. |
| Databáze: | OpenAIRE |
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