Glycyrrhizin, silymarin, and ursodeoxycholic acid regulate a common hepatoprotective pathway in HepG2 cells
| Autor: | Chien-Yun Hsiang, Li-Jen Lin, Shung Te Kao, Shun Ting Chou, Tin-Yun Ho, Hsin Yi Lo |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmaceutical Science
Apoptosis Biology Protective Agents Biological pathway chemistry.chemical_compound Drug Discovery Gene expression medicine Humans Glycyrrhizin Oligonucleotide Array Sequence Analysis Pharmacology Regulation of gene expression Ursodeoxycholic Acid NF-kappa B Lipid metabolism Hep G2 Cells Glycyrrhizic Acid Ursodeoxycholic acid Oxidative Stress Gene Expression Regulation Liver Complementary and alternative medicine chemistry Cancer research Molecular Medicine Tumor necrosis factor alpha Transcriptome Silymarin medicine.drug |
| Zdroj: | Phytomedicine. 22:768-777 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2015.05.053 |
| Popis: | Background Glycyrrhizin, silymarin, and ursodeoxycholic acid are widely used hepatoprotectants for the treatment of liver disorders, such as hepatitis C virus infection, primary biliary cirrhosis, and hepatocellular carcinoma. Purpose The gene expression profiles of HepG2 cells responsive to glycyrrhizin, silymarin, and ursodeoxycholic acid were analyzed in this study. Methods HepG2 cells were treated with 25 µM hepatoprotectants for 24 h. Gene expression profiles of hepatoprotectants-treated cells were analyzed by oligonucleotide microarray in triplicates. Nuclear factor-κB (NF-κB) activities were assessed by luciferase assay. Results Among a total of 30,968 genes, 252 genes were commonly regulated by glycyrrhizin, silymarin, and ursodeoxycholic acid. These compounds affected the expression of genes relevant various biological pathways, such as neurotransmission, and glucose and lipid metabolism. Genes involved in hepatocarcinogenesis, apoptosis, and anti-oxidative pathways were differentially regulated by all compounds. Moreover, interaction networks showed that NF-κB might play a central role in the regulation of gene expression. Further analysis revealed that these hepatoprotectants inhibited NF-κB activities in a dose-dependent manner. Conclusion Our data suggested that glycyrrhizin, silymarin, and ursodeoxycholic acid regulated the expression of genes relevant to apoptosis and oxidative stress in HepG2 cells. Moreover, the regulation by these hepatoprotectants might be relevant to the suppression of NF-κB activities. |
| Databáze: | OpenAIRE |
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