cDNA cloning and gene expression of the type 1 bovine interleukin-1 receptor
| Autor: | K Gosink, Charles J. Czuprynski, L.A Schuler, J.J Rejman, Matthew J. Beckman, S.S Giles |
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| Rok vydání: | 1999 |
| Předmět: |
Cell type
DNA Complementary Immunology Molecular Sequence Data Interleukin-1 receptor Biology Peripheral blood mononuclear cell Cell Line Mice Species Specificity Complementary DNA Gene expression medicine Coding region Animals Humans Amino Acid Sequence Cloning Molecular Fibroblast Receptor General Veterinary Base Sequence Reverse Transcriptase Polymerase Chain Reaction Receptors Interleukin-1 Molecular biology Rats medicine.anatomical_structure Cattle |
| Zdroj: | Veterinary immunology and immunopathology. 71(3-4) |
| ISSN: | 0165-2427 |
| Popis: | Regulation of interleukin-1 (IL-1) mediated biological responses is complicated by the multiple ligands and receptors of the IL-1 family. Most studies of IL-1 receptors have used human or rodent cells. Here, we report that the coding region of the bovine type 1 interleukin-1 receptor (type 1 IL-1R) cDNA extends 1719 bp in length. Northern analysis of specific bovine cell and tissue RNA demonstrated a 4.5 kb transcript. Overall, the bovine type 1 IL-1R coding region exhibits approximately 81 and 76% similarity with the human type 1 IL-1R at the nucleotide and amino acid level, respectively, and somewhat less similarity with the mouse and rat sequences. Type 1 IL-1R transcripts were confirmed by RT-PCR in several bovine cell types, including peripheral blood mononuclear cells (PBMCs), neutrophils (PMNs), and fibroblast, peritoneal macrophage, and arterial endothelial cell lines. It is expected that molecular clones for the bovine type 1 and 2 IL-1 receptors will provide us with the tools needed to decipher species-and cell-specific regulation of IL-1 action in the bovine. |
| Databáze: | OpenAIRE |
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