Minimal residual disease in acute myelogenous leukaemia and myelodysplastic syndromes: a follow-up of patients in clinical remission
| Autor: | Afsaneh Keyhani, Michael Andreeff, Angela Goodacre, Shuwei Jiang, Nguyen T. Van, Gisela Sanchez-Williams, Marek Kimmel, Heike Engel |
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| Rok vydání: | 1997 |
| Předmět: |
Oncology
Adult Male medicine.medical_specialty Monosomy Pathology Neoplasm Residual Proliferation index Aneuploidy Trisomy Biology Trisomy 8 Sensitivity and Specificity hemic and lymphatic diseases Internal medicine medicine Humans In Situ Hybridization Fluorescence Aged Aged 80 and over Chromosome Aberrations medicine.diagnostic_test Myelodysplastic syndromes Hematology Middle Aged medicine.disease Prognosis Minimal residual disease Survival Analysis Leukemia Myeloid Neoplasm Regression Spontaneous Karyotyping Myelodysplastic Syndromes Acute Disease Female Chromosomes Human Pair 7 Fluorescence in situ hybridization Chromosomes Human Pair 8 Follow-Up Studies |
| Zdroj: | British journal of haematology. 99(1) |
| ISSN: | 0007-1048 |
| Popis: | The majority of patients with acute myelogenous leukaemia (AML) and myelodysplastic syndromes (MDS) relapse, especially those with unfavourable cytogenetics. This study was designed to investigate the presence and frequency of minimal residual disease (MRD) in patients with AML or MDS (n = 35) and numerical abnormalities of chromosomes 6, 7, 8, 9, 10, 17 and 18 in clinical remission by using a combination of fluorescence activated cell sorting (FACS), fluorescence in-situ hybridization (FISH) and labelling with bromodeoxyuridine (BUdR). The technique enables the detection of as few as three leukaemic cells in 105 normal cells. MRD was detected in 33/35 patients in complete remission (CR). 16 patients relapsed (8/11 with monosomy 7, 4/17 with trisomy 8, and 4/7 with other cytogenetic abnormalities) after a median of 4.8 months (range 3–13). Levels of MRD (P = 0.007) and proliferation index (P = 0.011) were significantly higher in patients with monosomy 7 than in patients with trisomy 8 or other cytogenetic abnormalities. The percentage of cells in S-phase, the number of abnormal cells and cytogenetic class were related to time to relapse (P = 0.001) with S-phase being the single most important prognostic factor (P = 0.0001). We conclude that the combination of FACS/FISH/BUdR, which determines the number, phenotype and proliferation rate of very rare leukaemic cells in patients with AML or MDS in clinical remission, provides information that is useful in the identification of patients with high and low likelihood of relapse. |
| Databáze: | OpenAIRE |
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