Muscle tissue as an endocrine organ: Comparative secretome profiling of slow-oxidative and fast-glycolytic rat muscle explants and its variation with exercise
| Autor: | Arturo Roca-Rivada, Felipe F. Casanueva, Cecilia Castelao, Luisa M. Seoane, Tomás García-Caballero, María Pardo, Omar Al-Massadi, Lucia L. Senin, Jana Alonso |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Muscle tissue medicine.medical_specialty Time Factors Proteome Biophysics Muscle Proteins Biology Models Biological Biochemistry Rats Sprague-Dawley Gastrocnemius muscle Paracrine signalling Organ Culture Techniques Endocrine Glands Physical Conditioning Animal Internal medicine Myokine medicine Animals Electrophoresis Gel Two-Dimensional Secretion Autocrine signalling Cells Cultured Muscles Skeletal muscle Rats Endocrinology Secretory protein medicine.anatomical_structure Metabolome Energy Metabolism Glycolysis Oxidation-Reduction |
| Zdroj: | Journal of Proteomics. 75:5414-5425 |
| ISSN: | 1874-3919 |
| DOI: | 10.1016/j.jprot.2012.06.037 |
| Popis: | The notion that skeletal muscle is a secretory organ capable to release proteins that can act locally in an autocrine/paracrine manner or even in an endocrine manner to communicate with distant tissues has now been recognized. Under this context, a new paradigm has arisen implicating the muscle in metabolism regulation. Considering the evidences that give exercise a protective role against illnesses associated to physical inactivity, it becomes of especial relevance to characterize muscle secreted proteins. In the present study we show for the first time the secretome characterization and the comparative 2-DE secretome analysis among fast-glycolytic (gastrocnemius) and slow-oxidative (soleus) rat muscle explants and its variation after exercise intervention. We have identified 19 differently secreted proteins when comparing soleus and gastrocnemius secretomes, and 10 in gastrocnemius and 17 in soleus distinctive secreted proteins after 1 week of endurance exercise training. Among identified proteins, DJ-1 was found to be more abundant in fast-glycolytic fiber secretomes. On the contrary, FABP-3 was elevated in slow-oxidative fiber secretomes, although its secretion from gastrocnemius muscle increased in exercised animals. These and other secreted proteins identified in this work may be considered as potential myokines. |
| Databáze: | OpenAIRE |
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