Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris
| Autor: | Pratik Jaluria, Chiawei W. Tsai, David L. Narum, Je-Nie Phue, Joseph Shiloach, Loc Trinh |
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| Rok vydání: | 2006 |
| Předmět: |
Chromatography
biology Expanded bed adsorption Cell Culture Techniques Protozoan Proteins Bioengineering General Medicine Equipment Design Vaccine antigen biology.organism_classification Chromatography Ion Exchange Protein Engineering Applied Microbiology and Biotechnology Pichia Recombinant Proteins Pichia pastoris Distribution system Equipment Failure Analysis Malaria transmission Malaria Vaccines Protozoal disease Biotechnology |
| Zdroj: | Biotechnology letters. 28(13) |
| ISSN: | 0141-5492 |
| Popis: | An experimental malaria transmission blocking vaccine antigen, Pfs25H, expressed and secreted from Pichia pastoris was recovered and purified using a screenless expanded bed column equipped with a rotating fluid distribution system. This column was able to accommodate feed stock, containing 30% biomass, at a flow rate of 300-400 cm/h without affecting column stability. This capability is three times higher than the capability of the expanded bed column currently in use, which is equipped with a perforated plate fluid distribution system; this design could accommodate biomass concentrations of only up to 10%. The screen-less design did not affect the binding capacity, purification level or process yield and, therefore, shorten the process. Purified Pfs25H of 6.4 g were recovered from 37 l of Pichia pastoris culture in one step. |
| Databáze: | OpenAIRE |
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