IgE–Anti-IgE-Induced Prostaglandin D2Release from Cultured Human Mast Cells
| Autor: | Toru Obata, Kihei Maekawa, Takuroh Masaki, K. Yamashita, Masahiro Kanbe, Toshikazu Nagakura |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cell
Biophysics Prostaglandin Stem cell factor Stimulation Immunoglobulin E Biochemistry Umbilical cord Peripheral blood mononuclear cell Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Chymases medicine Humans Mast Cells Molecular Biology Cells Cultured biology Prostaglandin D2 Chemistry Serine Endopeptidases Cell Differentiation Cell Biology Fetal Blood Immunohistochemistry Molecular biology Antibodies Anti-Idiotypic medicine.anatomical_structure biology.protein Tryptases lipids (amino acids peptides and proteins) Cyclooxygenase |
| Zdroj: | Biochemical and Biophysical Research Communications. 225:1015-1020 |
| ISSN: | 0006-291X |
| Popis: | To elucidate the characterization of cultured human mast cells, the generation of prostaglandin D 2 (PGD 2 ) upon stimulation with IgE-anti IgE challenge was determined by using gas chromatography/mass spectrometry/selected ion monitoring (GC/MS/SIM) assay. Mononuclear cells obtained from human umbilical cord blood were cultured in the presence of Steel Factor, IL-6, and PGE 2 . After 8 weeks of culture, approximately 90 to 95% of cultured cells became tryptase-positive and included basophilic granules. At 12 weeks of culture, cells were harvested and incubated with 1 μg/ml of human IgE for 1 hr and then challenged with 10 μg/ml of anti-IgE. The level of PGD 2 release into the supernatant was measured by GC/MS/SIM with the stable isotope dilution method. Cyclooxygenase inhibitors affected the PGD 2 release from the cell by IgE-anti IgE challenge incubation. Indomethacin inhibited 98% of PGD 2 release. Acetylsalicylic acid (aspirin) and NS-398 (potent COX-2 inhibitor) also inhibited PGD 2 release by 85 and 45% respectively. |
| Databáze: | OpenAIRE |
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