The Drosophila ACP65A cuticle gene: deletion scanning analysis of cis-regulatory sequences and regulation by DHR38
| Autor: | Jean-Philippe Charles, Brigitte Quennedey, Matthieu Lestradet, A. Girard, Josiane Alabouvette, E. Gervasio, L. Hong, N. Bruey-Sedano |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Receptors Steroid Transcription Genetic Transgene lac operon Receptors Cytoplasmic and Nuclear Biology Fusion gene P element Animals Genetically Modified Endocrinology Genetics Nuclear Receptor Subfamily 4 Group A Member 1 Animals Drosophila Proteins Enhancer Gene Crosses Genetic Sequence Deletion Base Sequence Activator (genetics) fungi Pupa Cell Biology DNA-Binding Proteins Gene Expression Regulation Regulatory sequence Insect Proteins Drosophila Female Transcription Factors |
| Zdroj: | Genesis (New York, N.Y. : 2000). 43(1) |
| ISSN: | 1526-954X |
| Popis: | The regulatory sequences of the Drosophila ACP65A cuticle gene were analyzed in vivo in transgenic flies, using both fusion genes constructs and transposase-mediated deletions within a P element containing ACP65A regulatory sequences fused to the lacZ gene (deletion scanning). The sequences located between −594 and +161 are sufficient to confer both temporal and spatial expression specificities, indicating the presence of tissue-specific enhancers and response elements to hormone-induced factors. In addition, timing of expression and tissue-specificity appear to be controlled by distinct cis-regulatory elements, which suggests the existence of independent hormonal and tissue-specific signaling pathways. Gain and loss of function studies also implicate DHR38, the Drosophila homolog of the vertebrate NGFI-B-type nuclear receptors, as an important activator of the ACP65A gene. genesis 43:17–27, 2005. © 2005 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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