Comparison of HPLC and small column (CDTect) methods for disialotransferrin
| Autor: | Ulf-Håkan Stenman, Henrik Alfthan, Mikko Salaspuro, Taina Methuen, Ursula Turpeinen, Kalevi Laitinen |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Cost-Benefit Analysis Clinical Biochemistry Ion chromatography 01 natural sciences High-performance liquid chromatography 03 medical and health sciences Column chromatography Reference Values medicine Humans Protein Isoforms Chromatography High Pressure Liquid 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography medicine.diagnostic_test Chemistry 010401 analytical chemistry Biochemistry (medical) Area under the curve Transferrin Reproducibility of Results Chromatography Ion Exchange Confidence interval N-Acetylneuraminic Acid 0104 chemical sciences Alcoholism ROC Curve Immunoassay Quantitative analysis (chemistry) Biomarkers |
| Zdroj: | Clinical chemistry. 47(10) |
| ISSN: | 0009-9147 |
| Popis: | Background: Current methods for determination of carbohydrate-deficient transferrin (CDT) are based on separation of the CDT fraction by ion-exchange chromatography on minicolumns and quantification by immunoassay. Alternatively, the transferrin isoforms can be separated by HPLC anion-exchange chromatography and quantified by absorbance. This method has been reported to improve the validity of CDT as a marker of chronic alcohol abuse. Methods: HPLC on either MonoQ or ResourceQ anion-exchange columns was used to separate and quantify isoforms of transferrin with detection at 460 nm. The result was expressed as the percentage of the disialo form (pI 5.7) of total transferrin (DST). The commercial CDTectTM assay was used as a comparison method. Serum samples from nondrinkers (n = 57), moderate drinkers (n = 77), and heavy drinkers (n = 139) were analyzed. Results: In ROC analysis for differentiation between moderate and heavy drinkers, the area under the curve (AUC) for the HPLC method was 0.87 (95% confidence interval, 0.81–0.93), whereas that for CDTect was 0.72 (95% confidence interval, 0.64–0.80). At 90% specificity, the sensitivity of DST was 63% (95% confidence interval, 53–73%) compared with 33% (22–44%) for CDT. The reference interval of the HPLC method was 0.68–1.7%. Conclusions: The HPLC anion-exchange method for quantification of CDT provides substantially better separation between moderate and heavy drinkers than the CDTect method. |
| Databáze: | OpenAIRE |
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