Simultaneous Measurement of Serum Testosterone and Dihydrotestosterone by Liquid Chromatography–Tandem Mass Spectrometry
| Autor: | Ronald S. Swerdloff, Victor H.H. Goh, Steve Shiraishi, Christina Wang, Paul W. N. Lee, Andrew Leung |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Spectrometry Mass Electrospray Ionization Analyte medicine.medical_specialty medicine.drug_class Electrospray ionization Clinical Biochemistry Radioimmunoassay Mass spectrometry Sensitivity and Specificity Reference Values Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Internal medicine medicine Humans Testosterone Chromatography Chemistry Biochemistry (medical) Dihydrotestosterone Middle Aged Androgen Endocrinology Calibration Female Chromatography Liquid medicine.drug |
| Zdroj: | Clinical Chemistry. 54:1855-1863 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1373/clinchem.2008.103846 |
| Popis: | Background: Recent reports have described inherent problems with androgen immunoassays compared with mass spectrometry analyses. Methods: We developed a method for measuring serum testosterone (T) and 5α-dihydrotestosterone (DHT) simultaneously via liquid–liquid extraction followed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) with positive-mode electrospray ionization. Results: The DHT and T calibrators showed a linear response from 0.069 nmol/L to 34.4 nmol/L and 69.3 nmol/L, respectively. T interference in the DHT assay and vice versa were negligible. Within- and between-run imprecision values were Conclusions: We have developed and validated a selective and precise method for simultaneous measurements of serum T and DHT that can be adopted for routine measurements of these androgens in health and disease in men and women. |
| Databáze: | OpenAIRE |
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