Alteration in the Population of Intraepithelial Lymphocytes and Virus Shedding in Specific-Pathogen-Free Chickens Following Inoculation with Lentogenic and Velogenic Newcastle Disease Virus Strains

Autor:	Tasiu Mallam Hamisu, Hayatuddeen Bako Aliyu, Mohd Hair-Bejo, Abdul Rahman Omar, Aini Ideris
Rok vydání:	2022
Předmět:	Newcastle Disease Virology Immunology Newcastle disease virus Animals Molecular Medicine Viral Vaccines Chickens Intraepithelial Lymphocytes Poultry Diseases Virus Shedding
Zdroj:	Viral Immunology. 35:328-337
ISSN:	1557-8976 0882-8245
DOI:	10.1089/vim.2021.0148
Popis:	Intraepithelial lymphocytes (IELs) provide the first line of immunological defense after the invasion of the intestine by a pathogen. To understand the changes of IEL response in chickens, we measured the population of different subsets of avian IELs at different time points after primary inoculation of Newcastle disease virus (NDV) lentogenic strain (LaSota) and subsequent challenge with NDV velogenic strain- genotypes VII and VIII. Furthermore, NDV shed after each treatment was quantified. Specific-pathogen-free chickens were randomly divided into six groups of chickens, one to six, inoculated with phosphate buffered saline; NDV lentogenic strain (LaSota); genotype VII (GVII); LaSota and challenged with GVII (LSGVII); genotype VIII (GVIII); and group of LaSota and challenged with GVIII (LSGVIII). The chickens were euthanized at 12, 36, and 60 h postchallenge. Immunophenotyping of CD25
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eaf74caca86036516bc200168de20411 https://doi.org/10.1089/vim.2021.0148 Zobrazit plný text záznamu