Expression of endothelin precursor genes in human trophoblast in culture
| Autor: | André Malassiné, Thérèse-Marie Mignot, Paulette Duc-Goiran, Françoise Ferré, Laurent Cronier, Brigitte Robert, Christelle Bourgeois |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Molecular Sequence Data Gene Expression Biology Polymerase Chain Reaction Endocrinology Syncytiotrophoblast Internal medicine medicine Humans RNA Messenger Protein Precursors Cells Cultured reproductive and urinary physiology Messenger RNA Base Sequence Endothelin-1 Endothelins Trophoblast General Medicine Intervillous space Molecular biology Reverse transcriptase Trophoblasts Blotting Southern medicine.anatomical_structure Cell culture embryonic structures beta 2-Microglobulin Endothelin receptor Precursor mRNA |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1479-683X 0804-4643 |
| DOI: | 10.1530/eje.0.1340490 |
| Popis: | Robert B, Malassiné A, Bourgeois C, Mignot T-M, Cronier L, Ferré F, Duc-Goiran P. Expression of endothelin precursor genes in human trophoblast in culture. Eur J Endocrinol 1996;134:490–6. ISSN 0804–4643 We have shown previously the presence of immunoreactive endothelin in cultured trophoblastic cells from human term placenta as well as in the trophoblast-conditioned medium. To confirm whether or not the differentiated syncytiotrophoblast is a site for endothelin synthesis, we investigated, by reverse transcription and polymerase chain reaction, the expression of the three preproendothelin genes in 3-day cultured trophoblast. While no endothelin-2 precursor mRNA was detected, preproendothelin-1 mRNA was found to be expressed by the trophoblast. The endothelin-3 precursor gene was also expressed, but at low level and it was detected only after Southern blotting and oligonucleotide hybridization. The ability of trophoblast in culture to express the endothelin precursor genes supports the idea that, in human term placenta, villous syncytiotrophoblast that lines the intervillous space containing maternal blood acts as an endothelial layer. B Robert, U.361 INSERM, Maternité Baudelocque, 123 Bld de Port-Royal, 75014 Paris, France |
| Databáze: | OpenAIRE |
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