Proteomic Surveillance of Autoantigens in Relapsing Polychondritis
| Autor: | Manabu Nakamura, Toshihiro Matsui, Yasuhiko Tanaka, Tomohiro Kato, Hiroshi Nakamura, Hirobumi Kondo, Kusuki Nishioka, Kayo Masuko, Izumi Koizuka, Shigeto Tohma, Nobuko Iizuka, Kazuo Yudoh |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Proteomics Alpha-enolase Blotting Western Immunology Enzyme-Linked Immunosorbent Assay Vimentin medicine.disease_cause Autoantigens Microbiology Autoimmunity Antigen Tubulin Virology Biomarkers Tumor medicine Humans Electrophoresis Gel Two-Dimensional Polychondritis Relapsing Relapsing polychondritis Autoantibodies Glycoproteins biology Tumor Suppressor Proteins Autoantibody Middle Aged medicine.disease Molecular biology Recombinant Proteins DNA-Binding Proteins Blot Phosphopyruvate Hydratase Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Female Calreticulin Carrier Proteins |
| Zdroj: | Microbiology and Immunology. 50:117-126 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.2006.tb03776.x |
| Popis: | Relapsing polychondritis (RP) is a systemic inflammatory disease, in which autoimmunity to cartilage-related components is thought to be involved in its pathogenesis. However, the autoimmune profile in RP has not been studied fully. We therefore investigated autoantibodies/autoantigens in RP comprehensively, by 2-dimensional electrophoresis (2DE), subsequent western blotting (WB) and mass spectrometry, using cell-extracted proteins as the antigen source. As a result, we detected 15 autoantigens on 2DE-WB, and further identified five of them. On average, one RP serum recognized approximately 8 out of the 15 autoantigens. Frequencies of the autoantibodies to the 5 identified antigens of tubulin alpha ubiquitous/6, vimentin, alpha enolase, calreticulin, and colligin-1/-2 were 91%, 46%, 36%, 82%, and 36%, respectively. ELISA using recombinant proteins for them revealed that frequencies of the autoantibodies to tubulin alpha ubiquitous, vimentin, alpha enolase, calreticulin, and colligin-1 were 36%, 64%, 46%, 27%, and 18%, respectively. Our data demonstrated that the autoimmune reaction was not restricted to cartilage-related components, rather a variety of autoimmune responses occurred in patients with RP, which may be involved in the pathophysiology of RP. In addition, the proteomic approach using cell-extracted proteins would be a powerful way to investigate autoantigens. |
| Databáze: | OpenAIRE |
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