Structural and functional studies of vertebrate metallothioneins: Cross-talk between domains in the absence of physical contact
| Autor: | Daniela Di Maro, Elio Parisi, Piero Andrea Temussi, Orlando Crescenzi, Clemente Capasso, Roberta Spadaccini, Vincenzo Carginale |
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| Přispěvatelé: | Capasso, C., Carginale, V., Crescenzi, Orlando, Di Maro, D., Spadaccini, Roberta, Temussi, PIERO ANDREA, Parisi, E. |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Circular dichroism
Magnetic Resonance Spectroscopy FISH NOTOTHENIA-CORIICEPS Dithionitrobenzoic Acid Biochemistry CELLULAR ZINC Mice Species Specificity circular dichroism metallothionein metal mobility NMR thiol reactivity Notothenia coriiceps biology.animal Animals Metallothionein Sulfhydryl Compounds Functional studies Molecular Biology Phylogenetic tree biology STABILITY Chemistry Circular Dichroism CLUSTERS Fishes Temperature Vertebrate Biological Transport Cell Biology Glutathione Recombinant Proteins Protein Structure Tertiary Kinetics Zinc NMR-SPECTROSCOPY Biophysics Fish Oxidation-Reduction Cadmium Research Article |
| Zdroj: | Biochemical journal (Lond., 1984) 391 (2005): 95–103. doi:10.1042/BJ20050335 info:cnr-pdr/source/autori:Capasso C.; Carginale V.; Crescenzi O.; Di Maro D.; Spadaccini R.; Temussi P.A.; Parisi E./titolo:Structural and functional studies of vertebrate metallothioneins: Cross-talk between domains in the absence of physical contact/doi:10.1042%2FBJ20050335/rivista:Biochemical journal (Lond., 1984)/anno:2005/pagina_da:95/pagina_a:103/intervallo_pagine:95–103/volume:391 |
| DOI: | 10.1042/BJ20050335 |
| Popis: | In previous studies, we showed that the chemical and dynamic properties of fish and mouse MTs (metallothioneins) present a number of distinctive differences linked to their primary structures, and that phylogenetic relationships of mammal and fish MTs correlate with their three-dimensional structures. The different behaviours of MTs may also be linked to the interaction between their two domains. In the present study, we have compared the physicochemical properties of the isolated recombinant domains constituting Notothenia coriiceps and mouse MTs, and compared them with those of the corresponding whole MTs. NMR spectra of the separated domains of N. coriiceps are almost superimposable on those of the parent MT, suggesting an apparent lack of interaction between the two domains in the protein. However, certain dynamic and physicochemical features of the isolated domains are unlike those of the whole protein. In particular, the temperature-induced changes in the chiroptical properties, thiol reactivity of the Zn-MT domains and the Zn2+/Cd2+ rate of exchange are different for the two domains and with respect to the whole protein. Taken together, these results provide a strong argument in favour of the interaction of the two domains in the MT molecule, in spite of the elusive evidence provided by the structural analyses. |
| Databáze: | OpenAIRE |
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