Inhibition of Bacillus cereus phospholipase C by univalent anions
| Autor: | S E Aakre, C Little |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1982 |
| Předmět: |
Tris
Anions Sodium Phospholipid Bacillus cereus chemistry.chemical_element Phospholipase Sodium Chloride Biochemistry Medicinal chemistry chemistry.chemical_compound Hydrolysis Humans Formate Molecular Biology Phospholipids Phospholipase C biology Erythrocyte Membrane Cell Biology biology.organism_classification chemistry Phospholipases Type C Phospholipases Research Article |
| Popis: | The rate of phospholipid hydrolysis in erythrocyte ghosts by Bacillus cereus phospholipase C was markedly decreased by the presence of NaCl at concentrations between 25 and 200 mM. The inhibition seemed to be due to Cl- and was unaffected by the type of cation present. The larger univalent anions such as HCO3-, Br-, Cl-, NO3-, CNO- and I- seemed most effective, whereas the bivalent anion SO42- was relatively ineffective at 0.1 M, as were acetate and formate. Tris buffers at 0.1 M caused marked inhibition. With bovine brain myelin, phospholipid hydrolysis by phospholipase C was also much more strongly inhibited by I- and Cl- than by SO42- or acetate. NaCl inhibited the hydrolysis by the enzyme of the soluble substrate dihexanoylglycerophosphocholine, thereby suggesting that the inhibiton did not arise simply from substrate effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|