SPM-1-producing Pseudomonas aeruginosa ST277 clone recovered from microbiota of migratory birds

Autor: Stéfanie Vanessa Santos, Ana Cristina Gales, Patrícia Locosque Ramos, Ana Clara Narciso, Willames M. B. S. Martins, Lorena Cristina Corrêa Fehlberg, Rodrigo Cayô, João Batista da Cruz
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Polymyxin
Dendrocygna viduata
medicine.disease_cause
Antimicrobial resistance
Agar dilution
law.invention
law
Polymerase chain reaction
Polymyxin B
Environmental microbiology
Microbiota
General Medicine
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
Ducks
Infectious Diseases
Pseudomonas aeruginosa
Brazil
Bacterial Outer Membrane Proteins
DNA
Bacterial
Microbiology (medical)
Bacilli
medicine.drug_class
030106 microbiology
Porins
Microbial Sensitivity Tests
Biology
beta-Lactam Resistance
beta-Lactamases
Microbiology
Birds
Carbapenemase
03 medical and health sciences
Antibiotic resistance
Bacterial Proteins
Drug Resistance
Bacterial
Pulsed-field gel electrophoresis
medicine
Animals
Humans
Pseudomonas Infections
Bird Diseases
Membrane Transport Proteins
biology.organism_classification
Virology
Imipenem
Carbapenems
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Multilocus sequence typing
Animal Migration
Multilocus Sequence Typing
Zdroj: Repositório Institucional da UNIFESP
Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
Popis: Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) National Council for Science and Technological Development The production of Sao Paulo metallo-O-lactamase (SPM-1) is the most common carbapenem resistance mechanism detected among multidrug-resistant Pseudomonas aeruginosa clinical isolates in Brazil. Dissemination of SPM-1-producing P. aeruginosa has been restricted to the nosocomial settings, with sporadic reports of environmental isolates due to contamination by hospital sewage. Herein, we described the detection and molecular characterization of SPM-1-producing P. aeruginosa recovered from the microbiota of migratory birds in Brazil. Three hundred gram-negative bacilli were recovered from cloacal and choanal swabs of Dendrocygna viduata during a surveillance study for detection of carbapenem-resistant isolates. All isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. MICs were determined by agar dilution, except for polymyxin B. Antibiotic resistance genes were detected by polymerase chain reaction (PCR) followed by DNA sequencing. Transcriptional levels of oprD and efflux system encoding genes were also carried out by quantitative real-time PCR. Nine imipenem-resistant P. aeruginosa isolates were recovered with 7 of them carrying bla(SPM-1). Additional resistance genes (rmtD-1, bla(OXA-56), aacA4, and aac(6')-Ib-cr) were also detected in all 9 isolates. The SPM-1-producing isolates showed high MICs for all beta-lactams, fluoroquinolones, and aminoglycosides, being susceptible only to polymyxin B. Interestingly, all isolates showed the same PFGE pattern and belonged to ST277. Overexpression of MexXY-OprM and MexAB-OprM was observed in those isolates that did not harbor bla(SPM-1). Our results suggest that migratory birds might have played a role in the dissemination of SPM-1-producing P. aeruginosa within the Brazilian territory. (C) 2017 Elsevier Inc. All rights reserved. Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Internal Med, Div Infect Dis,Lab Alerta, Sao Paulo, Brazil Fundacao Parque Zool Sao Paulo, Dept Pesquisas Aplicadas, Sao Paulo, SP, Brazil FPZSP, Sao Paulo, Brazil Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Internal Med, Div Infect Dis,Lab Alerta, Sao Paulo, Brazil FAPESP: 2017/02258-6, 2014/12224-3 CAPES PNPD 20131991 National Council for Science and Technological Development: 305535/2014-5 Web of Science
Databáze: OpenAIRE
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