Borrelia burgdorferi sensu lato in Ixodes longiscutatus ticks from Brazilian Pampa
| Autor: | Ricardo Ott, Bruna Ferreira Leal, José Reck, José M. Venzal, Ugo Araújo Souza, Guilherme M. Klafke, Carlos A. Ferreira, Thais Michel, João Ricardo Martins, Bruno Dall’Agnol, Anelise Webster, Bárbara Weck |
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| Rok vydání: | 2017 |
| Předmět: |
Nymph
0301 basic medicine 030231 tropical medicine Borrelia afzelii medicine.disease_cause Microbiology 03 medical and health sciences 0302 clinical medicine Lyme disease Borrelia burgdorferi Group Borrelia mayonii Borrelia parasitic diseases medicine Animals Borrelia burgdorferi Phylogeny Ixodes biology Sequence Analysis DNA bacterial infections and mycoses biology.organism_classification medicine.disease Virology 030104 developmental biology Infectious Diseases Larva Insect Science Epidemiological Monitoring Female Parasitology Borrelia garinii Brazil Flagellin Borrelia spielmanii |
| Zdroj: | Repositório Institucional PUCRS Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
| ISSN: | 1877-959X |
| Popis: | Borrelia burgdorferi sensu lato (s.l.) complex includes the agents of Lyme disease/borreliosis in North America, Europe, and Asia, such Borrelia burgdorferi sensu stricto, Borrelia afzelii, Borrelia garinii, Borrelia bavariensis, Borrelia spielmanii, Borrelia bissettiae, and Borrelia mayonii. In 2013 B. burgdorferi s.l. was reported for the first time in the Neotropical region, from Ixodes aragaoi ticks in Uruguayan Pampa. In addition, from 2011 to 2016, 17 suspected human cases of borreliosis-like syndrome were reported in Rio Grande do Sul (RS) state, Brazil, which contains only part of country in the Pampa biome. The goal of this work is to report the results of a state surveillance program conducted in order to investigate the presence of B. burgdorferi s.l. in its classic vector, Ixodes spp. ticks, from the Brazilian Pampa. For this, we searched for Ixodes spp. ticks in 307 rodents from 11 municipalities of RS state. We then tested the ticks for the presence of B. burgdorferi s.l. DNA using PCR analysis. Of 35 Ixodes spp. ticks tested, one larva and one nymph of Ixodes longiscutatus ticks tested positive for Borrelia sp. DNA. The phylogenetic analysis of the flaB fragment grouped our samples (referred as Borrelia sp. haplotype Pampa) into B. burgdorferi s.l. group in a particular branch with other South American haplotypes, and this group was close to Borrelia carolinensis, B. bissettiae, and Borrelia californiensis. This is the first evidence of B. burgdorferi s.l. circulation in ticks of the genus Ixodes in Brazil. These results highlight the need for the implementation of public health policies for the diagnosis and prevention of potential cases of human borreliosis in Brazil. Further studies are needed to fill the gaps in our knowledge of the distribution, pathogenicity, reservoirs, and vectors of these emerging South American B. burgdorferi s.l. haplotypes. |
| Databáze: | OpenAIRE |
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