The COP9 signalosome is involved in the regulation of lipid metabolism and of transition metals uptake inSaccharomyces cerevisiae

Autor: Benedetta Mattei, Laylan Bramasole, Valerio Licursi, Chiara Salvi, Rodolfo Negri, Martin Bard, Giovanna Serino, Jacob Z. Zimbler, Claudia Fabbri, Brett M. Barnes, Teresa Rinaldi, Virginia De Cesare, Elah Pick
Přispěvatelé: Department of Biology and Biotechnology 'Charles Darwin', Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Department of Biology, Faculty of Sciences and Science Education, University of Haifa [Haifa], Department of Evolutionary and Environmental Biology, Department of Biology, Indiana University - Purdue University Indianapolis (IUPUI), Indiana University System-Indiana University System, This work was supported by FIRB 2011-2013 (grantno. RBIN06E9Z8) ‘Molecular Bases of Diseases’,PRIN 2009 ‘Role ofS. cerevisiaeGeneral RegulatoryFactors (GRF) in Chromatin Organization andDynamics’ and Progetti di Ricerca di Ateneo (grantno. C26A1139XY) to RN, Israel Ministry of Scienceand Technology (MOST)–Italy Ministry of ForeignAffairs (MAE) grant 3-9022 to RN, TR, GS and EP, and Israel Science Foundation grant (EP355/10) forEP, Progetto di Ricerca C26A1089CJ (2010), SapienzaUniversita’ di Roma, to RN, TR and GS. VL’s fellowship is supported by a grant from Regione Lazio, Università degli Studi di Roma 'La Sapienza' [Rome] - Réseau International des Instituts Pasteur - Institut Pasteur - Fondation Cenci Bolognetti, Indiana University - Purdue University
Rok vydání: 2013
Předmět:
medicine.disease_cause
MESH: Zinc
Biochemistry
MESH: Ergosterol
chemistry.chemical_compound
neddylation
MESH: Saccharomyces cerevisiae Proteins
cop9 signalosome
Ubiquitin
Tandem Mass Spectrometry
Ergosterol
MESH: Reverse Transcriptase Polymerase Chain Reaction
Transition Elements
Oligonucleotide Array Sequence Analysis
MESH: Lipid Metabolism
0303 health sciences
Mutation
biology
Reverse Transcriptase Polymerase Chain Reaction
MESH: Real-Time Polymerase Chain Reaction
MESH: Chromatography
Gas
030302 biochemistry & molecular biology
zinc uptake
Metalloendopeptidases
Cullin Proteins
MESH: Saccharomyces cerevisiae
Zinc
Cullin
Chromatography
Gas
Saccharomyces cerevisiae Proteins
MESH: Peptide Hydrolases
Protein subunit
Blotting
Western
ergosterol biosynthesis
lipid metabolism
Saccharomyces cerevisiae
MESH: Metalloendopeptidases
Real-Time Polymerase Chain Reaction
MESH: Cullin Proteins
MESH: Transition Elements
MESH: Gene Expression Profiling
03 medical and health sciences
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
medicine
MESH: Blotting
Western
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
RNA
Messenger
COP9 signalosome
Molecular Biology
Gene
MESH: RNA
Messenger
030304 developmental biology
COP9 Signalosome Complex
Gene Expression Profiling
MESH: Biological Markers
MESH: Tandem Mass Spectrometry
Cell Biology
MESH: Multiprotein Complexes
Lipid Metabolism
biology.organism_classification
chemistry
Multiprotein Complexes
MESH: Oligonucleotide Array Sequence Analysis
biology.protein
MESH: Chromatography
Liquid
Biomarkers
Chromatography
Liquid
Peptide Hydrolases
Zdroj: FEBS Journal
FEBS Journal, Wiley, 2013, 281 (1), pp.175-90. ⟨10.1111/febs.12584⟩
FEBS Journal, Wiley, 2013, 281 (1), pp.175-90. <10.1111/febs.12584>
ISSN: 1742-464X
1742-4658
DOI: 10.1111/febs.12584
Popis: International audience; The COP9 signalosome (CSN) is a highly conserved eukaryotic protein complex which regulates the cullin RING family of ubiquitin ligases and carries out a deneddylase activity that resides in subunit 5 (CSN5). Whereas CSN activity is essential for the development of higher eukaryotes, several unicellular fungi including the budding yeast Saccharomyces cerevisiae can survive without a functional CSN. Nevertheless, the budding yeast CSN is biochemically active and deletion mutants of each of its subunits exhibit deficiency in cullins deneddylation, although the biological context of this activity is still unknown in this organism. To further characterize CSN function in budding yeast, we present here a transcriptomic and proteomic analysis of a S. cerevisiae strain deleted in the CSN5/RRI1 gene (hereafter referred to as CSN5), coding for the only canonical subunit of the complex. We show that Csn5 is involved in modulation of the genes controlling amino acid and lipid metabolism and especially ergosterol biosynthesis. These alterations in gene expression correlate with the lower ergosterol levels and increased intracellular zinc content which we observed in csn5 null mutant cells. We show that some of these regulatory effects of Csn5, in particular the control of isoprenoid biosynthesis, are conserved through evolution, since similar transcriptomic and/or proteomic effects of csn5 mutation were previously observed in other eukaryotic organisms such as Aspergillus nidulans, Arabidopsis thaliana and Drosophila melanogaster. Our results suggest that the diverged budding yeast CSN is more conserved than was previously thought.
Databáze: OpenAIRE
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