Quantitative evaluation of regularized phase retrieval algorithms on bone scaffolds seeded with bone cells
| Autor: | Max Langer, Françoise Peyrin, S Tavella, Loriane Weber, Alessandra Ruggiu |
|---|---|
| Přispěvatelé: | European Synchrotron Radiation Facility (ESRF), Imagerie Tomographique et Radiothérapie, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Genova & Istituto Nazionale per la Ricerca sul Cancro, Universita degli studi di Genova |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Materials science
X-ray microtomography [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging 02 engineering and technology Regenerative medicine Bone and Bones 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Imaging Three-Dimensional regularized phase retrieval Bone cell Humans Radiology Nuclear Medicine and imaging bone scaffolds Tomographic reconstruction Radiological and Ultrasound Technology Tissue Scaffolds bone cells Soft tissue Anatomy X-Ray Microtomography 021001 nanoscience & nanotechnology holotomography quantification 3. Good health Seeding Tomography 0210 nano-technology Phase retrieval Porosity [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing Algorithms Biomedical engineering in-line phase contrast x-ray tomography |
| Zdroj: | Physics in Medicine and Biology Physics in Medicine and Biology, IOP Publishing, 2016, 61, pp.N215-N231. ⟨10.1088/0031-9155/61/9/N215⟩ |
| ISSN: | 0031-9155 1361-6560 |
| Popis: | International audience; In the field of regenerative medicine, there has been a growing interest in studying the combination of bone scaffolds and cells that can maximize newly formed bone. In-line phase-contrast x-ray tomography was used to image porous bone scaffolds (Skelite©), seeded with bone forming cells. This technique allows the quantification of both mineralized and soft tissue, unlikewith classical x-ray micro-computed tomography. Phase contrast images were acquired at four distances. The reconstruction is typically performed in two successive steps: phase retrieval and tomographic reconstruction. In this work, different regularization methods were applied to the phase retrieval process. The application of a priori terms for heterogeneous objects enables quantitative 3D imaging of not only bone morphology, mineralization, and soft tissue formation, but also cells trapped in the pre-bone matrix. A statistical study was performed to derive statistically significant information on the different culture conditions. |
| Databáze: | OpenAIRE |
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