Microbial Composition and Genes for Key Metabolic Attributes in the Gut Digesta of Sea Urchins Lytechinus variegatus and Strongylocentrotus purpuratus Using Shotgun Metagenomics
| Autor: | Casey D. Morrow, Asim K. Bej, George B. H. Green, Stephen A. Watts, Michael R. Crowley, Joseph A. Hakim |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
animal structures QH301-705.5 Ruegeria gut microbiome Zoology Microbiology biology.animal Gammaproteobacteria Biology (General) Molecular Biology Sea urchin Lytechinus variegatus MG-RAST biology urogenital system high-throughput sequencing bioinformatics General Medicine Roseobacter biology.organism_classification Photobacterium Strongylocentrotus purpuratus echinoderm Echinoderm KEGG RefSeq embryonic structures |
| Zdroj: | Current Issues in Molecular Biology Volume 43 Issue 2 Pages 70-995 Current Issues in Molecular Biology, Vol 43, Iss 70, Pp 978-995 (2021) |
| ISSN: | 1467-3045 |
| Popis: | This paper describes the microbial community composition and genes for key metabolic genes, particularly the nitrogen fixation of the mucous-enveloped gut digesta of green (Lytechinus variegatus) and purple (Strongylocentrotus purpuratus) sea urchins by using the shotgun metagenomics approach. Both green and purple urchins showed high relative abundances of Gammaproteobacteria at 30% and 60%, respectively. However, Alphaproteobacteria in the green urchins had higher relative abundances (20%) than the purple urchins (2%). At the genus level, Vibrio was dominant in both green (~9%) and purple (~10%) urchins, whereas Psychromonas was prevalent only in purple urchins (~24%). An enrichment of Roseobacter and Ruegeria was found in the green urchins, whereas purple urchins revealed a higher abundance of Shewanella, Photobacterium, and Bacteroides (q-value < 0.01). Analysis of key metabolic genes at the KEGG-Level-2 categories revealed genes for amino acids (~20%), nucleotides (~5%), cofactors and vitamins (~6%), energy (~5%), carbohydrates (~13%) metabolisms, and an abundance of genes for assimilatory nitrogen reduction pathway in both urchins. Overall, the results from this study revealed the differences in the microbial community and genes designated for the metabolic processes in the nutrient-rich sea urchin gut digesta, suggesting their likely importance to the host and their environment. |
| Databáze: | OpenAIRE |
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