Viable porcine hepatocytes from slaughterhouse organs
| Autor: | Schildberg Fw, Koebe S, Koebe Hg, J. Mueller-Hoecker |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Cell Survival Swine Biomedical Engineering Cell Separation Andrology chemistry.chemical_compound Coumarins Albumins medicine Animals Bile Urea Cells Cultured biology Albumin Cytochrome P450 Alanine Transaminase Metabolism DNA medicine.anatomical_structure Biochemistry chemistry Liver Lobe Liver Cell culture Hepatocyte biology.protein Female Digestion Abattoirs Biotechnology |
| Zdroj: | Artificial cells, blood substitutes, and immobilization biotechnology. 23(5) |
| ISSN: | 1073-1199 |
| Popis: | A modified enzymatic isolation technique for the successful harvesting of porcine liver cells from slaughterhouse organs is introduced. Digestion of the left medial liver lobe (n=74) resulted in 1.0 ±0.3 ± 10E7 viable hepatocytes per gram tissue and an overall yield of 1.92 ±0.5 ± 10E9 cells per isolation (viability: 93 ±2%).Morphological integrity of hepatocytes in long term immobilization culture systems was assessed by light and electron microscopic follow up. Stable DNA-contents and low alanineamino-transferase (ALAT) release were measured after early culture adaptation. Urea production under NH4Cl, Albumin secretion, total bile acid synthesis and a follow up of 7-ethoxicoumarin o-deethylase (ECOD) activity demonstrated functional activity and maintenance of Type IA1 cytochrome P450 (CYP450) dependent metabolism in cultured hepatocytes for at least 10 days.In contrast to published results, where hepatocytes have been mainly isolated from anaesthetized animals we could not see any disadvantages in the ... |
| Databáze: | OpenAIRE |
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