Quantification of Proviral Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR
| Autor: | Sophie Puyeo, Françoise Brun-Vézinet, Florence Damond, François Simon, Isabelle Farfara, Diane Descamps, Pauline Campa, Jean Noël Telles, Sophie Matheron, Annie Leprêtre |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Microbiology (medical) Cell HIV Infections Biology Sensitivity and Specificity Peripheral blood mononuclear cell Virus law.invention Proviruses law Virology medicine Humans Polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction Reproducibility of Results Middle Aged Viral Load Provirus biology.organism_classification CD4 Lymphocyte Count medicine.anatomical_structure Real-time polymerase chain reaction DNA Viral HIV-2 Lentivirus RNA Viral Female Viral load |
| Zdroj: | Journal of Clinical Microbiology. 39:4264-4268 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.39.12.4264-4268.2001 |
| Popis: | We have developed and evaluated a new method to quantify human immunodeficiency virus type 2 (HIV-2) proviral DNA based on LightCycler real-time PCR. The assay has a detection limit of 5 copies/10 5 peripheral blood mononuclear cells (PBMC) and is insensitive to HIV-2 strain variability: HIV-2 subtypes A and B are both recognized and quantified. The intra- and interassay coefficients of variation range from 16 to 40% for high provirus concentrations (5 × 10 5 copies) and from 41 to 39% for low concentrations (5 copies). We used this method to compare the proviral DNA load and viral RNA load in plasma with clinical and immunological status for 29 patients infected by HIV-2 (subtype A in 17 and subtype B in 12). The proviral load (median, 201 copies/10 5 PBMC) was similar to that reported for HIV-1 infection. The median proviral loads did not correlate with the CD4 + cell count categories and were as follows for CD4 + cell counts of >400, 200 to 400, and 3 , respectively: 121 copies/10 5 PBMC ( n = 8; range, 5 PBMC); 114 copies/10 5 PBMC ( n = 9; range, 5 PBMC); and 285 copies/10 5 PBMC ( n = 12; range, 53 to 2,524 copies/10 5 PBMC). Proviral load did not correlate with plasma HIV-2 RNA positivity. As HIV-2 is considered to replicate less efficiently than HIV-1, these high proviral loads might be explained by the proliferation of infected cells. |
| Databáze: | OpenAIRE |
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