Inhibition of Listeria monocytogenes , Escherichia coli O157:H7, and Micrococcus luteus by Linear Furanocoumarins in a Model Food System
| Autor: | Edmund A. Zottola, H. William Schafer, P. Michael Davidson, Jorge Ulate-Rodríguez |
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| Rok vydání: | 2019 |
| Předmět: |
Minimum bactericidal concentration
biology Micrococcus engineering.material biology.organism_classification medicine.disease_cause complex mixtures Microbiology law.invention Minimum inhibitory concentration Listeria monocytogenes law engineering Listeria medicine Food science Micrococcus luteus Essential oil Food Science Lime |
| Zdroj: | Journal of food protection. 60(9) |
| ISSN: | 1944-9097 |
| Popis: | Lime peel, parsnip, lemon peel, dried parsley flakes, cold pressed lime oil, and distilled lime oil samples were analyzed for the presence and concentration of the linear furanocoumarins (LFs) psoralen, 5-methoxypsoralen (5- MOP), and 8-methoxypsoralen (8-MOP) by thin layer chromatography and gas chromatography mass spectrometry. Cold-pressed lime oil had the highest LF content (psoralen, 67 ± 29 μg/ml, 5-MOP, 1,634 ± 62 μg/ml, and 8-MOP, 44 ± 2 μg/ml). The antimicrobial effectiveness of LFs against Listeria monocytogenes , Escherichia coli O157:H7, and Micrococcus luteus was tested in a model food system consisting of a slurry of 25% commercial "garden vegetables" baby food in 0.1% peptone water. Inhibition required UV activation after the addition of the LFs to the model system. Lime peel extract, cold-pressed lime oil, and a 5-MOP standard inhibited the growth of L. monocytogenes , but not E. coli O157:H7. M. luteus was inhibited only by the cold-pressed lime oil. The minimum LF concentration that caused inhibition of the growth of L. monocytogenes was 32 μg/g and the minimum bactericidal concentration was 43 μg/g. Cold-pressed lime oil inhibited L. monocytogenes even at the lowest concentration added to the model system (10 μg/g), while the corresponding LF standard did not. This suggested the presence of other antimicrobial agents in the oil. |
| Databáze: | OpenAIRE |
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