Discovery of Neolignan Glycosides with Acetylcolinesterase Inhibitory Activity from Huangjinya Green Tea Guided by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry Data and Global Natural Product Social Molecular Networking
| Autor: | Wei Wang, Jia-Ping Ke, Hao-Yue Wu, Guan-Hu Bao, Ya-Shuai Kong, Peng Zhang, Tie-Jun Ling |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Circular dichroism Nuclear Overhauser effect Mass spectrometry 01 natural sciences Camellia sinensis Lignans Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Humans Glycosides Spectroscopy Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Molecular Structure Tea Plant Extracts Chemistry 010401 analytical chemistry Glycoside General Chemistry 0104 chemical sciences Plant Leaves Kinetics Heteronuclear molecule Acetylcholinesterase Cholinesterase Inhibitors General Agricultural and Biological Sciences Two-dimensional nuclear magnetic resonance spectroscopy 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 67:11986-11993 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.9b05605 |
| Popis: | Global Natural Product Social feature-based networking was applied to follow the phytochemicals, including nine flavonoid glycosides, six catechins, and three flavonols in Huangjinya green tea. Further, a new 8-O-4'-type neolignan glycoside, camellignanoside A (1), and 15 known compounds (2-16) were isolated through a variety of column chromatographies, and the structure was elucidated extensively by ultra performance liquid chromatography-quadrupole-time-of-flight-tandem mass spectrometry, 1H and 13C nuclear magnetic resonance, heteronuclear single-quantum correlation, heteronuclear multiple-bond correlation, 1H-1H correlation spectroscopy, rotating frame nuclear Overhauser effect spectroscopy, and Nuclear Overhauser effect spectroscopy, and circular dichroism spectroscopies. Compounds 1 and 2 showed acetylcolinesterase inhibition activity, with IC50 = 0.75 and 0.18 μM, respectively. |
| Databáze: | OpenAIRE |
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