Induction of Apoptosis of Human Brain Microvascular Endothelial Cells by Shiga Toxin 1
| Autor: | Alisa K. Hughes, Donald E. Kohan, Zuhal Ergonul |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Programmed cell death
medicine.medical_treatment Globotriaosylceramide Apoptosis Biology Shiga Toxin 1 Proinflammatory cytokine chemistry.chemical_compound Shiga-like toxin medicine Humans Immunology and Allergy Annexin A5 Tumor Necrosis Factor-alpha Microcirculation Trihexosylceramides Brain Shiga toxin Endothelial stem cell Infectious Diseases Cytokine chemistry Immunology Cancer research biology.protein Tumor necrosis factor alpha Endothelium Vascular |
| Zdroj: | The Journal of Infectious Diseases. 187:154-158 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/345861 |
| Popis: | Brain injury is the most frequent cause of mortality among patients with the hemolytic-uremic syndrome. Human brain endothelial cells (HBECs) are resistant to Escherichia coli-derived Shiga toxin (Stx); however, inflammatory cytokines markedly increase HBEC sensitivity to Stx cytotoxicity. HBECs were exposed to tumor necrosis factor (TNF)-alpha, with and without Stx-1, and cell survival, (125)I-Stx1 binding, globotriaosylceramide content, cell necrosis, and cell apoptosis levels were determined. TNF greatly increased Stx-1 cytotoxicity, primarily through induction of apoptosis, in HBEC. |
| Databáze: | OpenAIRE |
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