Systemic and behavioral effects of intranasal administration of silver nanoparticles
Autor: | Mansi Krishan, Paul C. Howard, Charles V. Vorhees, Vinicius Carreira, Taylor Ingle, Bryan L. Eppert, Michael T. Williams, Laurie L. Davenport, Heidi Hsieh, Mary Beth Genter |
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Rok vydání: | 2015 |
Předmět: |
Male
Pathology medicine.medical_specialty Metal Nanoparticles Morris water navigation task Spleen Pharmacology Toxicology Article Silver nanoparticle Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Developmental Neuroscience In vivo Lateral Ventricles medicine Animals Tissue Distribution Lymphocytes Maze Learning Administration Intranasal Inhalation exposure Behavior Animal Dose-Response Relationship Drug Chemistry Silver Compounds Cell Differentiation Recognition Psychology Glutathione Mice Inbred C57BL medicine.anatomical_structure Gene Expression Regulation Choroid Plexus Heme Oxygenase (Decyclizing) Toxicity Nasal administration |
Zdroj: | Neurotoxicology and Teratology. 51:68-76 |
ISSN: | 0892-0362 |
Popis: | Use of silver nanoparticles (AgNPs) for their antimicrobial properties is widespread. Much of the previous work on the toxicity of AgNPs has been conducted in vitro or following oral or intravenous administration in vivo. Intranasal (IN) instillation of AgNPs mimics inhalation exposure and allows further exploration of the toxicity of these particles via respiratory tract exposure. The present study involved 1) single-dose exposures to assess tissue distribution and toxicity and 2) repeated exposures to assess behavioral effects of IN AgNP exposure (nominally uncoated 25 nm AgNP). AgNP deposition was localized in the liver, gut-associated lymphoid tissue, and brain. Decrease cellularity in spleen follicles was observed in treated mice, along with changes in cell number and populations in the spleen. The splenic GSH:GSSG ratio was also reduced following AgNP exposure. Expression of the oxidative stress-responsive gene Hmox1 was elevated in the hippocampus, but not cortex of treated mice, as was the level of HMOX1 protein. Mice receiving 7 days of IN exposure to 50 mg/kg AgNPs exhibited similar learning- and memory-related behaviors to control mice, except that treated mice spent significantly less time in the target quadrant of the Morris Water Maze during the acquisition phase probe trial. These findings indicate systemic distribution and toxicity following IN administration of AgNPs. |
Databáze: | OpenAIRE |
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