Rotavirus Inner Capsid VP6 Acts as an Adjuvant in Formulations with Particulate Antigens Only
| Autor: | Kirsi Tamminen, Vesa P. Hytönen, Maria Malm, Suvi Heinimäki, Vesna Blazevic |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
nanostructure medicine.medical_treatment T cell viruses Immunology P particle lcsh:Medicine Coxsackievirus medicine.disease_cause Article Microbiology 03 medical and health sciences 0302 clinical medicine Immune system Antigen adjuvant Drug Discovery medicine Influenza A virus Pharmacology (medical) Pharmacology biology Chemistry Immunogenicity lcsh:R virus diseases biology.organism_classification peptide 030104 developmental biology Infectious Diseases medicine.anatomical_structure Capsid alum M2e VP6 Adjuvant 030215 immunology |
| Zdroj: | Vaccines Vaccines, Vol 8, Iss 365, p 365 (2020) Volume 8 Issue 3 |
| ISSN: | 2076-393X |
| Popis: | Novel adjuvants present a concern for adverse effects, generating a need for alternatives. Rotavirus inner capsid VP6 protein could be considered a potential candidate, due to its ability to self-assemble into highly immunogenic nanospheres and nanotubes. These nanostructures exhibit immunostimulatory properties, which resemble those of traditional adjuvants, promoting the uptake and immunogenicity of the co-administered antigens. We have previously elucidated an adjuvant effect of VP6 on co-delivered norovirus and coxsackievirus B1 virus-like particles, increasing humoral and cellular responses and sparing the dose of co-delivered antigens. This study explored an immunostimulatory effect of VP6 nanospheres on smaller antigens, P particles formed by protruding domain of a norovirus capsid protein and a short peptide, extracellular matrix protein (M2e) of influenza A virus. VP6 exhibited a notable improving impact on immune responses induced by P particles in immunized mice, including systemic and mucosal antibody and T cell responses. The adjuvant effect of VP6 nanospheres was comparable to the effect of alum, except for induction of superior mucosal and T cell responses when P particles were co-administered with VP6. However, unlike alum, VP6 did not influence M2e-specific immune responses, suggesting that the adjuvant effect of VP6 is dependent on the particulate nature of the co-administered antigen. |
| Databáze: | OpenAIRE |
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