Rhizobium leguminosarum exoB mutants are deficient in the synthesis of UDP-glucose 4'-epimerase
| Autor: | J W Redmond, C. A. Wijffelman, L Eydems, M.W. Breedveld, M Batley, E. Pees, H.C.J. Canter Cremers, L P Zevehuizen, Ben J. J. Lugtenberg |
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| Přispěvatelé: | Faculteit Medische Wetenschappen/UMCG |
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
UDP-glucose 4-epimerase
Rhizobiaceae Magnetic Resonance Spectroscopy Genotype Mutant Molecular Sequence Data Oligosaccharides medicine.disease_cause Biochemistry Rhizobium leguminosarum Microbiology chemistry.chemical_compound UDPglucose 4-Epimerase Plasmid medicine Journal Article Symbiosis Molecular Biology biology Research Support Non-U.S. Gov't Genetic Complementation Test food and beverages Cell Biology biochemical phenomena metabolism and nutrition biology.organism_classification chemistry Carbohydrate Sequence Genes Bacterial Galactose Mutation Rhizobium bacteria Bacteria Plasmids |
| Zdroj: | The Journal of Biological Chemistry, 265(34), 21122-21127. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| Popis: | Rhizobium leguminosarum bv. viciae Exo- mutant strains RBL5523,exo7::Tn5,RBL5523,exo8::Tn5 and RBL5523,exo52::Tn5 are affected in nodulation and in the syntheses of lipopolysaccharide, capsular polysaccharide, and exocellular polysaccharide. These mutants were complemented for nodulation and for the syntheses of these polysaccharides by plasmid pMP2603. The gene in which these mutants are defective is functionally homologous to the exoB gene of Rhizobium meliloti. The repeating unit of the residual amounts of EPS still made by the exoB mutants of R. leguminosarum bv. viciae lacks galactose and the substituents attached to it. The R. leguminosarum bv. viciae and R. meliloti exoB mutants fail to synthesize active UDP-glucose 4'-epimerase. |
| Databáze: | OpenAIRE |
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