Assessment of biological activity and UPLC–MS based chromatographic profiling of ethanolic extract of Ochradenus arabicus
| Autor: | Joongku Lee, Khaled M. Al-Anazi, Zeid A. ALOthman, M. Abul Farah, M. Ajmal Ali, Faisal M. Abou-Tarboush, Fahad M.A. Al-Hemaid, Saikh Mohammad Wabaidur |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cytotoxicity Pharmacology medicine.disease_cause Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine MTT assay Viability assay Chromatography Agricultural and Biological Sciences(all) ROS Biological activity Glutathione Ochradenus arabicus 030104 developmental biology UPLC–MS chemistry Phytochemical Biochemistry Oxidative stress 030220 oncology & carcinogenesis Original Article General Agricultural and Biological Sciences |
| Zdroj: | Saudi Journal of Biological Sciences. 23:229-236 |
| ISSN: | 1319-562X |
| DOI: | 10.1016/j.sjbs.2015.02.010 |
| Popis: | Natural products from wild and medicinal plants, either in the form of crude extracts or pure compounds provide unlimited opportunities for new drug leads owing to the unmatched availability of chemical diversity. In the present study, the cytotoxic potential of crude ethanolic extract of Ochradenus arabicus was analyzed by MTT cell viability assay in MCF-7 adenocarcinoma breast cancer cells. We further investigated its effect against oxidative stress induced by anticancer drug doxorubicin. In addition, Ultra Performance Liquid Chromatography–Mass Spectrometry (UPLC–MS) based chromatographic profiling of crude extract of O. arabicus was performed. The MTT assay data showed that the extract is moderately toxic to the MCF-7 cells. However, its treatment alone does not induce oxidative stress while doxorubicin increases the level of oxidative stress in MCF-7 cells. Whereas, simultaneous treatment of plant extract and doxorubicin significantly (p |
| Databáze: | OpenAIRE |
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