Methylation of Subtelomeric Chromatin Modifies the Expression of the lncRNA TERRA, Disturbing Telomere Homeostasis

Autor: Diego Oliva-Rico, Eunice Fabian-Morales, Rodrigo E. Cáceres-Gutiérrez, Adriana Gudiño, Fernanda Cisneros-Soberanis, Julieta Dominguez, Oscar Almaraz-Rojas, Cristian Arriaga-Canon, Clementina Castro-Hernández, Carlos De la Rosa, José L. Reyes, Luis A. Herrera
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Molecular Sciences; Volume 23; Issue 6; Pages: 3271
Oliva-Rico, D, Fabian-Morales, E, Cáceres-Gutiérrez, R E, Gudiño, A, Cisneros-Soberanis, F, Dominguez, J, Almaraz-Rojas, O, Arriaga-Canon, C, Castro-Hernández, C, De la Rosa, C, Reyes, J L & Herrera, L A 2022, ' Methylation of subtelomeric chromatin modifies the expression of the lncRNA TERRA, disturbing telomere homeostasis ', International Journal of Molecular Sciences, vol. 23, no. 6, 3271 . https://doi.org/10.3390/ijms23063271
ISSN: 1422-0067
DOI: 10.3390/ijms23063271
Popis: The long noncoding RNA (lncRNA) telomeric repeat-containing RNA (TERRA) has been associated with telomeric homeostasis, telomerase recruitment, and the process of chromosome healing; nevertheless, the impact of this association has not been investigated during the carcinogenic process. Determining whether changes in TERRA expression are a cause or a consequence of cell transformation is a complex task because studies are usually carried out using either cancerous cells or tumor samples. To determine the role of this lncRNA in cellular aging and chromosome healing, we evaluated telomeric integrity and TERRA expression during the establishment of a clone of untransformed myeloid cells. We found that reduced expression of TERRA disturbed the telomeric homeostasis of certain loci, but the expression of the lncRNA was affected only when the methylation of subtelomeric bivalent chromatin domains was compromised. We conclude that the disruption in TERRA homeostasis is a consequence of cellular transformation and that changes in its expression profile can lead to telomeric and genomic instability.
Databáze: OpenAIRE
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