Isolation and Localization of Mesenchymal Stem Cells in Human Palatine Tonsil by W5C5 (SUSD2)
| Autor: | Ji Min Kim, Byung-Joo Lee, Sung-Chan Shin, Dae-Woon Kang, Jin-Choon Lee, Jeon-Yeob Jang, Soo-Geun Wang, Jin Sup Jung, Hee-Young Park, Ji-Sun Song |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Stromal cell Adolescent Physiology Cellular differentiation Palatine Tonsil Biology Palatine tonsil lcsh:Physiology lcsh:Biochemistry 03 medical and health sciences SUSD2 Antigen Antigens CD medicine Humans lcsh:QD415-436 Mesenchymal stem cells Tonsil W5C5 Localization Child Cells Cultured Cell Proliferation Membrane Glycoproteins lcsh:QP1-981 Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Immunohistochemistry 030104 developmental biology medicine.anatomical_structure Child Preschool Stem cell |
| Zdroj: | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY(38): 1 Cellular Physiology and Biochemistry, Vol 38, Iss 1, Pp 83-93 (2016) |
| ISSN: | 1421-9778 |
| Popis: | Background/Aims: Although tonsil-mesenchymal stem cells (T-MSCs) have been studied as a new autologous or homologous source of MSCs, research on specific markers of MSCs and localization for purified T-MSC isolation has not yet been reported. This study investigates the expression of W5C5 (SUSD2) in tonsil stromal cells and the colony-forming ability and differentiation potential of W5C5+ cells to determine the usefulness of W5C5+ MSCs as a marker that can be used for the purification of T-MSCs. In addition, the location of W5C5+ cells expressed in the tonsil tissues is examined. Methods: T-MSCs were isolated from the tonsillar tissues of 12 patients undergoing tonsillectomy. The colony-forming ability, surface markers, proliferation potential, and differentiation capacities of purified W5C5+ MSCs, W5C5-MSCs, and unselected T-MSCs were evaluated. The location of the W5C5+ cells in the tonsillar tissues was also investigated by immunohistochemistry. Results: W5C5 was expressed in 2.5 +/- 0.4% of fresh human tonsil stromal cells. W5C5+ cells formed many colonies, but W5C5-cells did not form any colonies. The colony-forming number of W5C5+ cells (74.4 +/- 9.8) was significantly higher than that of unselected tonsil stromal cells (23.6 +/- 3.7). However, the differences in proliferation potential, surface marker expression, and differentiation potential between W5C5+ T-MSCs and unselected T-MSCs were not significant. W5C5+ cells were identified in the perivascular area around the blood vessels. Conclusion: W5C5+ T-MSCs possessed typical MSC properties with high colony-forming efficiency, and niches of W5C5+ T-MSCs were located in the perivascular area of tonsil tissues. These findings suggest that W5C5 is a useful single marker for the isolation of purified T-MSCs. (C) 2016 The Author(s) Published by S. Karger AG, Basel |
| Databáze: | OpenAIRE |
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