Trans10,cis15 18:2 Isolated from Beef Fat Does Not Have the Same Anti-Adipogenic Properties as Trans10,cis12-18:2 in 3T3-L1 Adipocytes
| Autor: | Payam Vahmani, Michael E. R. Dugan, William J. Meadus, David C. Rolland, Pascale Duff |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Conjugated linoleic acid Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound Mice Functional Food Adipocyte 3T3-L1 Cells Adipocytes Animals Linoleic Acids Conjugated Fatty acid synthesis Triglycerides chemistry.chemical_classification ACACA 030109 nutrition & dietetics Adipogenesis Lipogenesis Organic Chemistry Fatty Acids Fatty acid Cell Biology Red Meat 030104 developmental biology chemistry Fatty acid elongation lipids (amino acids peptides and proteins) Cattle Anti-Obesity Agents Hydrogenation |
| Zdroj: | Lipids. 51(11) |
| ISSN: | 1558-9307 |
| Popis: | During ruminal biohydrogenation of α-linolenic acid, a non-conjugated non-methylene interrupted dienoic acid is formed containing a t10 double bond, namely t10,c15-18:2. The present study was designed to examine whether t10,c15-18:2 would exert similar anti-adipogenic effects compared to t10,c12-18:2 in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were treated with 35 or 70 µM of LNA, t10,c12-18:2, t10,c15-18:2, or bovine serum albumin (BSA) vehicle control for 120 h. Cellular triacylglycerol and protein were quantified using commercial colorimetric kits. Cells were analyzed for fatty acid composition and gene expression using gas chromatography and quantitative PCR, respectively. Trans10,cis12-18:2 decreased (P |
| Databáze: | OpenAIRE |
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