Genomics of the new species Kingella negevensis: diagnostic issues and identification of a locus encoding a RTX toxin
| Autor: | Trestan Pillonel, Gilbert Greub, Guy Prod'hom, Sacha Laurent, Marie Léger, Onya Opota, Katia Jaton, Sabrina Trachsel |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Neisseriaceae Infections Bacterial Toxins 030106 microbiology Immunology Genomics Locus (genetics) Biology Real-Time Polymerase Chain Reaction Microbiology DNA sequencing Young Adult 03 medical and health sciences RNA Ribosomal 16S Humans Gene Phylogeny Comparative genomics Bacterial Toxins/genetics Female Kingella/genetics Kingella/isolation & purification Neisseriaceae Infections/diagnosis Neisseriaceae Infections/genetics Neisseriaceae Infections/microbiology RNA Ribosomal 16S/genetics Vaginosis Bacterial/microbiology Hemolysin proteins Infection Kingella kingae Matrix-assisted laser desorption-ionization mass spectrometry Real-time polymerase chain reaction RTX toxin Hemolysin Vaginosis Bacterial biology.organism_classification Virology 030104 developmental biology Infectious Diseases Kingella |
| Zdroj: | Microbes and infection, vol. 19, no. 11, pp. 546-552 |
| Popis: | Kingella kingae, producing the cytotoxic RTX protein, is a causative agent of serious infections in humans such as bacteremia, endocarditis and osteoarticular infection, especially in young children. Recently, Kingella negevensis, a related species, has been isolated from the oral cavity of healthy children. In this study, we report the isolation of K. negevensis strain eburonensis, initially misidentified as K. kingae with MALDI-TOF MS, from a vaginal specimen of a patient suffering of vaginosis. The genome sequencing and analysis of this strain together with comparative genomics of the Kingella genus revealed that K. negevensis possesses a full homolog of the rtx operon of K. kingae involved in the synthesis of the RTX toxin. We report that a K. kingae specific diagnostic PCR, based on the rtxA gene, was positive when tested on K. negevensis strain eburonensis DNA. This cross-amplification, and risk of misidentification, was confirmed by in silico analysis of the target gene sequence. To overcome this major diagnostic issue we developed a duplex real-time PCR to detect and distinguish K. kingae and K. negevensis. In addition to this, the identification of K. negevensis raises a clinical issue in term of pathogenic potential given the production of a RTX hemolysin. |
| Databáze: | OpenAIRE |
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