Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts : A Method to Characterize the Gastrointestinal Stem Cell Niche
| Autor: | Patrick Kollmann, Agnieszka Pastula, Gemma Mazzuoli-Weber, Moritz Tobiasch, Steffi Neupert, Ihsan Ekin Demir, Michael Quante, Moritz Middelhoff, Bettina Höhl, Anna Brandtner, Andreas H. Nuber |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
lcsh:Internal medicine Pathology medicine.medical_specialty Article Subject Cell- och molekylärbiologi Cell Biology Biology Gastrointestinal epithelium In vitro Cell biology ddc 03 medical and health sciences 030104 developmental biology In vivo Cell culture Organoid medicine Enteric nervous system Stem cell lcsh:RC31-1245 Molecular Biology Ex vivo Cell and Molecular Biology Research Article |
| Zdroj: | Stem Cells International Stem Cells International, Vol 2016 (2016) |
| Popis: | The gastrointestinal epithelium is characterized by a high turnover of cells and intestinal stem cells predominantly reside at the bottom of crypts and their progeny serve to maintain normal intestinal homeostasis. Accumulating evidence demonstrates the pivotal role of a niche surrounding intestinal stem cells in crypts, which consists of cellular and soluble components and creates an environment constantly influencing the fate of stem cells. Here we describe different 3D culture systems to culture gastrointestinal epithelium that should enable us to study the stem cell nichein vitroin the future: organoid culture and multilayered systems such as organotypic cell culture and culture of intestinal tissue fragmentsex vivo. These methods mimic thein vivosituationin vitroby creating 3D culture conditions that reflect the physiological situation of intestinal crypts. Modifications of the composition of the culture media as well as coculturing epithelial organoids with previously described cellular components such as myofibroblasts, collagen, and neurons show the impact of the methods applied to investigate niche interactionsin vitro. We further present a novel method to isolate labeled nerves from the enteric nervous system using Dclk1-CreGFP mice. |
| Databáze: | OpenAIRE |
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