Histone deacetylase 1 is required for Carbamazepine-induced CYP3A4 expression
| Autor: | Xiaopeng Shi, Jinwen Wang, Xianzhi Zhang, Yonghong Liu, Xiao-Xing Luo, Aidong Wen, Yin Wu |
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| Rok vydání: | 2012 |
| Předmět: |
Receptors
Steroid Clinical Biochemistry Pharmaceutical Science Histone Deacetylase 1 Pharmacology Steroid biosynthesis Analytical Chemistry Drug Discovery Tumor Cells Cultured Cytochrome P-450 CYP3A Humans Gene silencing Inducer Gene Silencing RNA Small Interfering Promoter Regions Genetic Receptor Spectroscopy Pregnane X receptor CYP3A4 biology Chemistry Pregnane X Receptor Cytochrome P450 Hep G2 Cells HDAC1 DNA-Binding Proteins Carbamazepine Hepatocytes biology.protein Anticonvulsants Protein Binding |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 58:78-82 |
| ISSN: | 0731-7085 |
| Popis: | Carbamazepine (CBZ) is a commonly prescribed antiepileptic drug. Adverse effects and drug-drug interaction are the two major concerns for its clinic application. CBZ is mainly metabolized by cytochrome P450 (CYP) 3A4, a strong inducer of CYP3A4 as well, which in turn influences the pharmaceutical profiles of the co-administrated drugs. To date, little is known about the mechanisms underlying CBZ-induced CYP3A4 expression. In this study, we explored the possible roles of Pregnane X receptor (PXR) and the histone deacetylase 1 (HDAC1) on the CBZ-induced CYP3A4 expression. The results showed that: (1) Although the expression of PXR was increased in CBZ treated cells, PXR gene silencing surprisingly showed no significant effects on CBZ-induced CYP3A4 expression; (2) CBZ inhibited the binding of HDAC1 to the promoter of CYP3A4. In addition, both dominant negative form and siRNA of HDAC1 could repress the CBZ-induced CYP3A4 expression. These data, for the first time indicate that HDAC1, is required for the CBZ-induced CYP3A4 expression. |
| Databáze: | OpenAIRE |
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