In vitro and in silico analyses of Vicia faba L. on Peroxisome proliferator-activated receptor gamma
| Autor: | V. Devi Rajeswari, D. Sathya Prabhu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Models Molecular In silico Peroxisome proliferator-activated receptor Biochemistry Models Biological Cell Line 03 medical and health sciences chemistry.chemical_compound Chalcones medicine Animals Hypoglycemic Agents Computer Simulation Apigenin Molecular Biology Butein chemistry.chemical_classification Dose-Response Relationship Drug Plant Extracts food and beverages Cell Biology Peroxisome Vicia faba Rats Up-Regulation Molecular Docking Simulation PPAR gamma 030104 developmental biology chemistry Mechanism of action Quercetin medicine.symptom |
| Zdroj: | Journal of cellular biochemistry. 119(9) |
| ISSN: | 1097-4644 |
| Popis: | The agonists of peroxisome proliferator-activated receptor gamma (PPARγ) from natural victual products were used as antidiabetic agents. Faba bean (Vicia faba L.) is a consequential legume that was known to possess potential antidiabetic activity, whose mechanism of action was unknown. The current study was focused to ascertain gene expression of the nuclear receptor PPARγ by Faba bean pod extract in rat cell lines (RINm5F).The real-time polymerase chain reaction analysis demonstrated that Faba bean pod extract in concentrations of 160 µg/mL have shown 4.97-fold stimulation compared with control. The cells treated with 320 µg/mL has shown 5.89-fold upregulation, respectively. Furthermore, in silico docking analysis was carried out against PPARγ, using the bioactive compounds identified from Faba bean pod extracts, which were known reported compounds from the literature. The results suggest that gene expression of PPARγ was inhibited by the constituents in Faba bean. In silico analysis prognosticates, butein has a high binding energy (-8.6 kcal/mol) with an atomic contact energy of -214.10, followed by Apigenin and Quercetin against PPARγ. Similarly, the percentage of interaction was high for butein, followed by Apigenin and Quercetin than other compounds comparatively. Hence, the results conclude inhibition of PPARγ by the bioactive compounds from Faba bean, which may provide insights into developing future therapeutic molecules for diabetes mellitus. |
| Databáze: | OpenAIRE |
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