Depletion of Neutrophils Exacerbates the Early Inflammatory Immune Response in Lungs of Mice Infected with Paracoccidioides brasiliensis
| Autor: | Martha Eugenia Urán-Jiménez, Ángel González, Paula Andrea Pino-Tamayo, Damaris Lopera, Juan David Puerta-Arias |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lung Diseases Male Chemokine Neutrófilos Article Subject Neutrophils medicine.medical_treatment Immunology Inflammation Paracoccidioides Proinflammatory cytokine 03 medical and health sciences Mice Immune system lcsh:Pathology medicine Animals Lung Paracoccidioides brasiliensis Mice Inbred BALB C biology Paracoccidioidomycosis Antibodies Monoclonal Cell Biology biology.organism_classification medicine.disease 030104 developmental biology Cytokine biology.protein Paracoccidioidomicosis Cytokines medicine.symptom Chemokines lcsh:RB1-214 Ratones Endogámicos BALB C Research Article |
| Zdroj: | Mediators of Inflammation Repositorio UdeA Universidad de Antioquia instacron:Universidad de Antioquia Mediators of Inflammation, Vol 2016 (2016) |
| ISSN: | 0962-9351 |
| DOI: | 10.1155/2016/3183285 |
| Popis: | Neutrophils predominate during the acute phase of theParacoccidioides brasiliensisinfection. Herein, we determined the role of the neutrophil during the early stages of experimental pulmonary paracoccidioidomycosis using a monoclonal antibody (mAb) specific for neutrophils. Male BALB/c mice were inoculated intranasally with1.5×106or2×106P. brasiliensisyeast cells. The mAb was administered 24 h before infection, followed by doses every 48 h until mice were sacrificed. Survival time was evaluated and mice were sacrificed at 48 h and 96 h after inoculation to assess cellularity, fungal load, cytokine/chemokine levels, and histopathological analysis. Neutrophils from mAb-treated mice were efficiently depleted (99.04%). Eighty percent of the mice treated with the mAb and infected with1.5×106yeast cells died during the first two weeks after infection. When mice were treated and infected with2×106yeast cells, 100% of them succumbed by the first week after infection. During the acute inflammatory response significant increases in numbers of eosinophils, fungal load and levels of proinflammatory cytokines/chemokines were observed in the mAb-treated mice. We also confirmed that neutrophils are an important source of IFN-γand IL-17. These results indicate that neutrophils are essential for protection as well as being important for regulating the early inflammatory immune response in experimental pulmonary paracoccidioidomycosis. |
| Databáze: | OpenAIRE |
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