Design, characterization and preclinical efficacy of a cationic lipid adjuvant for influenza split vaccine

Autor: Elisabeth Lafay-Vialon, Jean Haensler, Nicole Pascal, Sophie Gimenez, Emanuelle Trannoy, Annabelle Bonnin, Bruno Guy, Alain Francon
Rok vydání: 2001
Předmět:
Influenza vaccine
Chemistry
Pharmaceutical
Injections
Subcutaneous
medicine.medical_treatment
Static Electricity
Orthomyxoviridae
Drug Evaluation
Preclinical
Hemagglutinin Glycoproteins
Influenza Virus
Glycerophospholipids
Antibodies
Viral
Interferon-gamma
Mice
Adjuvants
Immunologic
Antigen
Cations
Animals
Outbred Strains
medicine
Animals
Humans
Particle Size
Immunity
Mucosal
Administration
Intranasal
Mice
Inbred BALB C
Liposome
General Veterinary
General Immunology and Microbiology
biology
Phosphatidylethanolamines
Immunogenicity
technology
industry
and agriculture
Public Health
Environmental and Occupational Health
Haplorhini
Hemagglutination Inhibition Tests
biology.organism_classification
Virology
Vaccination
Cholesterol
Infectious Diseases
Influenza Vaccines
Drug Design
Liposomes
Phosphatidylcholines
Molecular Medicine
Female
lipids (amino acids
peptides
and proteins)
Nasal administration
Adjuvant
T-Lymphocytes
Cytotoxic
Zdroj: Vaccine. 19:1794-1805
ISSN: 0264-410X
DOI: 10.1016/s0264-410x(00)00386-8
Popis: We prepared a series of cationic lipid vesicles comprising a cationic cholesterol derivative, DC-Chol with or without a neutral phospholipid, DOPC or DOPE. The vesicles were tested for their ability to bind and adjuvant split inactivated influenza vaccines. We found that DC-Chol-containing liposomes are capable to strongly bind influenza vaccine antigens upon simple mixing with the vaccine. The resulting formulations induced robust anti-influenza immune responses both after s.c. and i.n. administration in BALB/c mice while neutral Cholesterol/DOPC liposomes displayed virtually no stable antigen binding and no adjuvant effect. The parenteral adjuvant effect of DC-Chol on trivalent split influenza vaccines was then confirmed in outbred mice and monkeys. Among the most potent formulations tested, a simple mixture of the vaccine with a microfluidized dispersion of DC-Chol in an aqueous buffer is being considered for further development to produce an improved influenza vaccine.
Databáze: OpenAIRE
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