Advanced Material Catheter (AMCath), a minimally invasive endocardial catheter for the delivery of fast-gelling covalently cross-linked hyaluronic acid hydrogels
| Autor: | Aiden Flanagan, Gabriella Bellavia, Vladimir Velebny, Martin Pravda, Gerard M. Cooney, Stefania Straino, Romana Sulakova, Eimear B. Dolan, Ivana Ščigalková, Dorothee Daro, Garry P. Duffy, Nathalie Braun, Hugh O'Neill, Helena M. Kelly, Bruce P. Murphy, Lenka Kovarova, Brenton Cavanagh |
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| Přispěvatelé: | Seventh Framework Programme |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Swine
Biomedical Engineering Injectable hydrogels Biocompatible Materials macromolecular substances 02 engineering and technology minimally invasive delivery 030204 cardiovascular system & hematology Regenerative medicine complex mixtures Cardiac Catheters Cell Line Injections Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Delivery Systems In vivo Hyaluronic acid hyaluronic acid Animals Humans Chemistry Stem Cells technology industry and agriculture Hydrogels Equipment Design catheter Cells Immobilized 021001 nanoscience & nanotechnology Catheter Hydrogel Cross-Linking Reagents myocardial infarction Self-healing hydrogels Stem cell Left ventricle wall 0210 nano-technology Biomedical engineering Stem Cell Transplantation |
| Popis: | Injectable hydrogels that aim to mechanically stabilise the weakened left ventricle wall to restore cardiac function or to deliver stem cells in cardiac regenerative therapy have shown promising data. However, the clinical translation of hydrogel-based therapies has been limited due to difficulties injecting them through catheters. We have engineered a novel catheter, Advanced Materials Catheter (AMCath), that overcomes translational hurdles associated with delivering fast-gelling covalently cross-linked hyaluronic acid hydrogels to the myocardium. We developed an experimental technique to measure the force required to inject such hydrogels and determined the mechanical/viscoelastic properties of the resulting hydrogels. The preliminary in vivo feasibility of delivering fast-gelling hydrogels through AMCath was demonstrated by accessing the porcine left ventricle and showing that the hydrogel was retained in the myocardium post-injection (three 200 μL injections delivered, 192, 204 and 183 μL measured). However, the mechanical properties of the hydrogels were reduced by passage through AMCath (≤20.62% reduction). We have also shown AMCath can be used to deliver cardiopoietic adipose-derived stem cell-loaded hydrogels without compromising the viability (80% viability) of the cells in vitro. Therefore, we show that hydrogel/catheter compatibility issues can be overcome as we have demonstrated the minimally invasive delivery of a fast-gelling covalently cross-linked hydrogel to the beating myocardium. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: AMCARE consortium (FP7/2007-2013) under Grant Agreement No.604531. peer-reviewed |
| Databáze: | OpenAIRE |
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