Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear
| Autor: | Stefan R. O. Stolle, Pooyan Aliuos, Markus Schomaker, Athanasia Warnecke, Thomas Lenarz, J Schulze, Tammo Ripken, Günter Reuter, Darja Werner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
inner ear lcsh:Medicine drug implant animal cell nanobead Mice 0302 clinical medicine Drug Delivery Systems Animal Cells Medicine and Health Sciences Cytotoxic T cell magnetic bead drug delivery system Electron Microscopy animal genetics Internalization lcsh:Science Cell Analysis Dewey Decimal Classification::500 | Naturwissenschaften Connective Tissue Cells media_common Drug Implants Microscopy Multidisciplinary atomic force microscopy cochlea prosthesis Pharmaceutics Chemistry Physics Adhesion Anatomy Condensed Matter Physics fibroblast culture unclassified drug Bioassays and Physiological Analysis Connective Tissue brain derived neurotrophic factor Physical Sciences Drug delivery Magnets cytotoxicity ddc:500 Cellular Types Research Article Cell Viability Testing in vitro study NIH 3T3 cell line Cell Survival media_common.quotation_subject Materials Science Research and Analysis Methods Article 03 medical and health sciences Magnetics bright field microscopy In vivo Animals controlled study ddc:610 Viability assay Cell adhesion Materials by Attribute cell viability mouse nonhuman Scanning Probe Microscopy Brain-Derived Neurotrophic Factor lcsh:R Biology and Life Sciences cell adhesion Cell Biology Fibroblasts 3T3 cell line In vitro internalization Biological Tissue Magnetic Fields 030104 developmental biology Cochlear Implants Ears Bright Field Imaging drug effects magnetism Ear Inner Biophysics NIH 3T3 Cells Transmission Electron Microscopy lcsh:Q Dewey Decimal Classification::600 | Technik::610 | Medizin Gesundheit Drug Delivery Head 030217 neurology & neurosurgery cell structure |
| Zdroj: | PLoS ONE, Vol 11, Iss 2, p e0150057 (2016) PLoS ONE 11 (2016), Nr. 2 PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Introduction Long-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant surfaces with cells need to be developed. Our vision is to make benefit of electromagnetic field attracting forces generated by CI electrodes to bind BDNF-secreting cells that are labelled with magnetic beads (MB) onto the electrode surfaces. Thus, the effect of MB-labelling on cell viability and BDNF production were investigated. Materials and Methods Murine NIH 3T3 fibroblasts-genetically modified to produce BDNF-were labelled with MB. Results Atomic force and bright field microscopy illustrated the internalization of MB by fibroblasts after 24 h of cultivation. Labelling cells with MB did not expose cytotoxic effects on fibroblasts and allowed adhesion on magnetic surfaces with sufficient BDNF release. Discussion Our data demonstrate a novel approach for mediating enhanced long-term adhesion of BDNF-secreting fibroblasts on model electrode surfaces for cell-based drug delivery applications in vitro and in vivo. This therapeutic strategy, once transferred to cells suitable for clinical application, may allow the biological modifications of CI surfaces with cells releasing neurotrophic or other factors of interest. © 2016 Aliuos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|