Expression of IGF-I and IGF-binding protein genes in cirrhotic liver
| Autor: | S L Chew, L D'Souza Li, J. Rodriguez-Arnao, Cecilia Camacho-Hübner, Richard J. Ross, Jeffrey M P Holly, Alexander Gimson, M Yateman |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Liver Cirrhosis Male medicine.medical_specialty Cirrhosis Adolescent Endocrinology Diabetes and Metabolism medicine.medical_treatment Blotting Western Gene Expression In situ hybridization Biology Endocrinology Internal medicine Gene expression medicine Humans Regeneration RNA Messenger Northern blot Insulin-Like Growth Factor I Child In Situ Hybridization Messenger RNA Protease Radioimmunoassay Middle Aged Blotting Northern medicine.disease Insulin-Like Growth Factor Binding Protein 1 Insulin-Like Growth Factor Binding Proteins Blot Insulin-Like Growth Factor Binding Protein 2 Insulin-Like Growth Factor Binding Protein 3 Electrophoresis Polyacrylamide Gel Female hormones hormone substitutes and hormone antagonists Peptide Hydrolases |
| Zdroj: | Journal of Endocrinology. 149:209-216 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.1490209 |
| Popis: | The liver plays a central role in the IGF-I axis producing the majority of circulating hormone and some of its binding proteins (IGFBPs). Cirrhosis of the liver is characterised by changes in IGF-I and IGFBPs associated with liver fibrosis and regeneration. We have studied steady state levels of mRNA for the genes in the IGF-I axis in normal and cirrhotic human liver, localised the most highly expressed gene, IGFBP-1, and measured circulating IGFBP-3 by radioimmunoassay (RIA), IGFBP-2 and IGFBP-3 by Western ligand blot (WLB), and protease activity for IGFBP-3 in cirrhotic patients. Messenger RNA for IGF-I, IGFBP-1, IGFBP-2, and IGFBP-3 was detectable by Northern blotting in normal and cirrhotic liver although there was considerable variation in expression. IGFBP-2 and IGFBP-3 tended to be more highly expressed in cirrhotic liver and IGFBP-1 was more highly expressed in normal liver, although there were no significant differences. In normal liver, in situ hybridisation localised IGFBP-1 to hepatocytes. In cirrhotic liver the regenerating nodules showed expression of IGFBP-1 while there was none in fibrotic tissue. Circulating IGFBP-3 levels were low as measured by RIA and WLB but protease activity was only found in one patient. IGFBP-2 levels, assessed by WLB, were similar to the normal serum pool. Our data show that key mRNAs involved in the IGF-I axis continue to be expressed in cirrhotic liver despite end stage liver disease. The low levels of IGFBP-3 do not appear to be due to reduced gene transcription or increased protease activity. Journal of Endocrinology (1996) 149, 209–216 |
| Databáze: | OpenAIRE |
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